| Detailed Information for Phage Lonelysoil |
| Discovery Information |
| Isolation Host | Microbacterium foliorum NRRL B-24224 |
| Found By | Shyla Sherman |
| Year Found | 2021 |
| Location Found | Spokane, WA United States |
| Finding Institution | Gonzaga University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 23°C |
| GPS Coordinates | 47.667347 N, 117.403352 W Map |
| Discovery Notes | lonely soil was found outside of Hughs Hall on Gonzaga's campus. It was found under a tree in dry dirt. |
| Naming Notes | The phage's name comes from the loss of a partner. Mid -semester my partner dropped and therefore had to continue on by myself. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Feb 8, 2022 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 728 |
| Genome length (bp) | 61378 |
| Character of genome ends | Direct Terminal Repeat |
| Direct Terminal Repeat Length | 199 bp |
| GC Content | 66.9% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | EG |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | Gonzaga University |
| Annotation Status | In GenBank |
| Plaque Notes | Plaques were quite small and cloudy around the edge. They look like when you poke a sharpie on a paper and the ink continues to bleed. |
| Morphotype | Siphoviridae |
| Number of Genes | 105 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | OR253915 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
144 |
| Pitt Freezer Box Grid# |
H9 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
