| Detailed Information for Phage Lucky3 |
| Discovery Information |
| Isolation Host | Microbacterium foliorum NRRL B-24224 |
| Found By | Kerry Iles |
| Year Found | 2017 |
| Location Found | Pittsburgh, PA USA |
| Finding Institution | University of Pittsburgh |
| Program | Phage Hunters Integrating Research and Education |
| From enriched soil sample? | No |
| Isolation Temperature | Not entered |
| GPS Coordinates | 40.262021 N, 79.565175 W Map |
| Discovery Notes | This phage was isolated from the plant Trifolium repens, which was collected by Elizabeth M. O’Neill from the Ashman lab. The plant was washed with PYCa, filtered, and then enriched with host at 30 degrees Celsius. Purification and amplification was completed by Kira Zack. |
| Naming Notes | Trifolium repens is the white clover, which are considered lucky and generally have 3 leaves. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Nov 14, 2017 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 3441 |
| Genome length (bp) | 39640 |
| Character of genome ends | Circularly Permuted |
| GC Content | 64.1% |
| Sequencing Notes | originally sequenced as Golden, renamed Lucky3 |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | EA |
| Subcluster | EA4 |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | University of Pittsburgh |
| Annotation Status | In GenBank |
| Plaque Notes | Plaques are clear and have variable morphologies--small and large plaques. |
| Morphotype | Siphoviridae |
| Number of Genes | 58 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MG925347 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
8 |
| Pitt Freezer Box Grid# |
A2 |
| Available Files |
| Plaque Picture | Download |
| Final DNAMaster File | Download |
| GenBank File for Phamerator | Download |
