| Detailed Information for Phage MillyPhilly |
| Discovery Information |
| Isolation Host | Microbacterium foliorum NRRL B-24224 |
| Found By | Almas Thaha , Tracy Marcelis |
| Year Found | 2018 |
| Location Found | Philadelphia, PA USA |
| Finding Institution | Drexel University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 23°C |
| GPS Coordinates | 39.9598 N, 75.1904 W Map |
| Discovery Notes | When collecting the soil, an area of the soil touching the root was taken as well as part of to root. The only obstacle present while collecting the soil was the damp texture of the soil due to the rainfall that took place an hour beforehand. Therefore the soil collected was arranged in clumps rich in water, and parts of the root of the tree. |
| Naming Notes | The phage was named MillyPhilly because it was found outside of a building called "Millennium Hall" on Drexel University's campus which is located in Philadelphia. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 2, 2019 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 1455 |
| Genome length (bp) | 41800 |
| Character of genome ends | Circularly Permuted |
| GC Content | 63.5% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | EA |
| Subcluster | EA1 |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | Drexel University |
| Annotation Status | In GenBank |
| Plaque Notes | The plaque morphology of this phage has varied throughout isolation and purification. Originally having a more temperate morphology, the image of the plaques show a more clear morphology, but in more recent assays the plaques have been temperate again. The pure population of this phage has multiple morphologies with varying sizes. In the image shown, the sizes include plaques with diameters of 2mm, 1.5mm, and 1mm. The pure population was verified. |
| Morphotype | Siphoviridae |
| Number of Genes | 63 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MK620903 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
82 |
| Pitt Freezer Box Grid# |
A3 |
| Available Files |
| Plaque Picture | Download |
| GenBank File for Phamerator | Download |
