| Discovery Information |
| Isolation Host | Microbacterium foliorum NRRL B-24224 |
| Found By | Kayla Sweeney and Will Grillo |
| Year Found | 2019 |
| Location Found | Philadelphia, PA United States |
| Finding Institution | University of the Sciences (now St. Joseph's Univ) |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 24°C |
| GPS Coordinates | 39.945333 N, 75.207056 W Map |
| Discovery Notes | In the University of the Sciences Flower Garden located in the Quad area, next to the path. About a foot away from foot traffic and about fifty feet from the Wilson Dining Hall. Seems to have been recently mulched. |
| Naming Notes | Originally had a different name, but was taken in the data base.
Mr. Worldwide inspiration came from a helpful Teaching Assistant that was always willing to be a guide during the phage isolation process. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Dec 10, 2019 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 560 |
| Genome length (bp) | 41810 |
| Character of genome ends | Circularly Permuted |
| GC Content | 63.6% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | EA |
| Subcluster | EA1 |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | University of Southern California |
| Annotation Status | In GenBank |
| Plaque Notes | Plaques appeared to be extremely small and fill the entire plate. Plaques appeared to be very turbid and cloudy, with a small bullseye in the middle of the plaques when closely looked at on the bacterial lawn. |
| Morphotype | Siphoviridae |
| Number of Genes | 63 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | OR521079 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
1.8*10^8 |
| Pitt Freezer Box# |
98 |
| Pitt Freezer Box Grid# |
D1 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
