| Detailed Information for Phage Nicky22 |
| Discovery Information |
| Isolation Host | Microbacterium foliorum NRRL B-24224 |
| Found By | Camila Lomanto |
| Year Found | 2022 |
| Location Found | Rosario, Argentina |
| Finding Institution | Universidad Nacional de Rosario |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 19°C |
| GPS Coordinates | 32.94 S, 60.652 W Map |
| Discovery Notes | This phage was isolated from a soil sample. The sample contained grass, dry leaves, small rocks and insects as well as dirt. It seemed like the soil had recently been watered. |
| Naming Notes | The sample from which the phage was isolated was collected from an emblematic boulevard located at the heart of Rosario city. The boulevard was named after Nicasio Oroño, an ex gevernor of our province. That is the reason why I chose Nicky as the phage's name and the following 22 means the year of isolation 2022. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 19, 2023 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Approximate Shotgun Coverage | 697 |
| Genome length (bp) | 41785 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CTTCCGGCAT |
| GC Content | 66.8% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | EB |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | Universidad Nacional de Rosario |
| Annotation Status | In GenBank |
| Plaque Notes | the phage forms clear, circular, big plaques of about 4 mm of diameter with regular margens. |
| Morphotype | Siphoviridae |
| Number of Genes | 69 |
| Number of tRNAs | 1 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | OR613468 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Not in Pitt Archives |
| Available Files |
| Plaque Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
