Microbacterium phage QMacho
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Detailed Information for Phage QMacho
Discovery Information
Isolation HostMicrobacterium foliorum NRRL B-24224
Found ByBrian Camacho
Year Found2021
Location FoundCharlotte, NC USA
Finding InstitutionQueens University of Charlotte
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature28°C
GPS Coordinates35.189512 N, 80.831178 W Map
Discovery NotesThe bacteriophage was found by a tree near Queens University of Charlotte during a sunny morning.
Naming NotesMy last name is Camacho, and my lab partner who I worked with during this phage experiment's last name is Quigley, so we combined the last names.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 4, 2022
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage548
Genome length (bp)41685
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceTCTCCCGGCA
GC Content67.0%
Fasta file available?Yes: Download fasta file
Characterization
ClusterEB
Subcluster--
Cluster Life CycleLytic
Other Cluster Members
Annotating InstitutionQueens University of Charlotte
Annotation StatusIn GenBank
Plaque NotesMedium sized and clear.
MorphotypeSiphoviridae
Number of Genes70
Number of tRNAs3
Number of tmRNAs0
Has been Phamerated?Yes
Gene List

Click to View

QMacho_1

QMacho_2

QMacho_3
(terminase small subunit)

QMacho_4
(terminase large subunit)

QMacho_5
(portal protein)

QMacho_6
(capsid maturation protease)

QMacho_7
(major capsid protein)

QMacho_8

QMacho_9
(head-to-tail adaptor)

QMacho_10
(head-to-tail stopper)

QMacho_11

QMacho_12
(tail terminator)

QMacho_13
(major tail protein)

QMacho_14
(tail assembly chaperone)

QMacho_15
(tail assembly chaperone)

QMacho_16

QMacho_17
(tape measure protein)

QMacho_18
(minor tail protein)

QMacho_19
(minor tail protein)

QMacho_20

QMacho_21
(minor tail protein)

QMacho_22
(minor tail protein)

QMacho_23
(endolysin)

QMacho_24

QMacho_25

QMacho_26

QMacho_27

QMacho_28

QMacho_29

QMacho_30
(Cas4 family exonuclease)

QMacho_31

QMacho_32

QMacho_33
(MazG-like nucleotide pyrophosphohydrolase)

QMacho_34
(thymidylate kinase)

QMacho_35
(recombination directionality factor)

QMacho_36

QMacho_37

QMacho_38
(NrdH-like glutaredoxin)

QMacho_39
(holliday junction resolvase)

QMacho_40

QMacho_41

QMacho_42

QMacho_43

QMacho_44
(DNA primase/helicase)

QMacho_45
(DNA polymerase I)

QMacho_46

QMacho_47

QMacho_48
(DNA binding protein)

QMacho_49

QMacho_50

QMacho_51
(ParB-like nuclease domain protein)

QMacho_52

QMacho_53

QMacho_54
(HNH endonuclease)

QMacho_55

QMacho_56

QMacho_57

QMacho_58

QMacho_59
(dihydrofolate reductase)

QMacho_60

QMacho_61
(thymidylate synthase)

QMacho_62

QMacho_63

QMacho_64

QMacho_65

QMacho_66

QMacho_67

QMacho_68

QMacho_69 — tRNA
(tRNA-Asn(gtt))

QMacho_70 — tRNA
(tRNA-Gln(ctg))

QMacho_71 — tRNA
(tRNA-Lys(ctt))

QMacho_72
(HNH endonuclease)

QMacho_73

Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionOP434443
Refseq NumberNone yet
Archiving Info
Archiving status Not in Pitt Archives
SEA Lysate Titer 2.3 x 10^9 pful/ml
Available Files
Plaque PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload