| Detailed Information for Phage Rapheph |
| Discovery Information |
| Isolation Host | Microbacterium foliorum NRRL B-24224 |
| Found By | Rafef Elsaid, Ariannah Lowe, and Juliana Buccieri |
| Year Found | 2022 |
| Location Found | Upper Darby Township, PA United States of America |
| Finding Institution | Drexel University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 23°C |
| GPS Coordinates | 39.96393 N, 75.27934 W Map |
| Discovery Notes | The soil was dug on September 20th, 2022 at 9:27 pm. The sample was collected from the base of a plant in the shade, in samp soil that was dug 2-3 inches deep. the general location was Upper Darby Philadelphia.
the soil collection, direct and enriched isolation, initial plaque assay, and spot test was completed by Rafef Elsaid.
The phage was then adopted by lab partners, Juliana Buccieri and Ariannah Lowe who began working with the phage at the purification stage. |
| Naming Notes | Juliana and Ariannah named their given phage Rapheph after the student, Rafef Elsaid who so kindly donated their phage so that the pair could continue their research. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 2, 2023 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Approximate Shotgun Coverage | 1939 |
| Genome length (bp) | 41805 |
| Character of genome ends | Circularly Permuted |
| GC Content | 63.6% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | EA |
| Subcluster | EA1 |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | Drexel University |
| Annotation Status | In GenBank |
| Plaque Notes | The plaque we purified had bullseyes throughout and was cloudy on the edges but clear in the center. The plaques were not a definite circle shape but rather rough around the edges. |
| Number of Genes | 63 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | OR613481 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
161 |
| Pitt Freezer Box Grid# |
H10 |
| Available Files |
| Plaque Picture | Download |
| GenBank File for Phamerator | Download |
