| Detailed Information for Phage SadLad |
| Discovery Information |
| Isolation Host | Microbacterium paraoxydans NRRL B-14843 |
| Found By | Margaret Marx |
| Year Found | 2019 |
| Location Found | New Richmond, WI United States |
| Finding Institution | University of Wisconsin-River Falls |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 28°C |
| GPS Coordinates | 45.2 N, 92.5 W Map |
| Discovery Notes | Found in a home vegetable garden. |
| Naming Notes | Due to many repeated procedures, I often found myself to be a sad lad. So I decided to share that burden with my sweet little phage. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | May 8, 2020 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 71 |
| Genome length (bp) | 62801 |
| Character of genome ends | Direct Terminal Repeat |
| Direct Terminal Repeat Length | 211 bp |
| GC Content | 69.0% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | EG |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | Austin Peay State University |
| Annotation Status | In GenBank |
| Plaque Notes | small, clear, no halo, variable |
| Morphotype | Siphoviridae |
| Number of Genes | 103 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MW924641 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
102 |
| Pitt Freezer Box Grid# |
H4 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
