| Discovery Information |
| Isolation Host | Microbacterium foliorum NRRL B-24224 |
| Found By | Brittany McGlone, Caroline Virone, Sadia Ali |
| Year Found | 2018 |
| Location Found | Philadelphia, PA USA |
| Finding Institution | University of the Sciences (now St. Joseph's Univ) |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 21°C |
| GPS Coordinates | 39.94749 N, 75.20695 W Map |
| Discovery Notes | The soil sample was collected outside of the PharmTox Center at University of the Sciences in Philadelphia. With Starbucks coffee in hand, the sample was taken from a planting box full of flowers and other plants and was about 2 cm deep in moist soil. |
| Naming Notes | Dating back to the olden days, people often referred to inanimate, genderless objects as "she/her" (think old ships). We have continued this in our daily loves, calling everything "she/her". When we discovered our phage, we thought it would be fitting to continue the tradition. Thus, Shee was born |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 8, 2019 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 3961 |
| Genome length (bp) | 41800 |
| Character of genome ends | Circularly Permuted |
| GC Content | 63.5% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | EA |
| Subcluster | EA1 |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | University of the Sciences (now St. Joseph's Univ) |
| Annotation Status | In GenBank |
| Plaque Notes | The phage appears to be temperate as evident by the turbid/bullseye plaques. On plates of higher concentration, the plaques appeared small and turbid, but when they were on lower concentrations they spread out and formed bullseyes.
UPDATE: After sending Shee for sequencing, we have learned our phage is classified as lytic. Her plaques remain a mystery! |
| Morphotype | Siphoviridae |
| Number of Genes | 63 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MK919471 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
4.4*10^9 |
| Pitt Freezer Box# |
84 |
| Pitt Freezer Box Grid# |
E7 |
| Available Files |
| Plaque Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
