| Detailed Information for Phage TeddyBear |
| Discovery Information |
| Isolation Host | Microbacterium foliorum NRRL B-24224 |
| Found By | Ann-Catherine Stanton |
| Year Found | 2016 |
| Location Found | Pittsburgh, PA USA |
| Finding Institution | University of Pittsburgh |
| Program | Phage Hunters Integrating Research and Education |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 40.4464 N, 79.9544 W Map |
| Discovery Notes | Found in soil outside Langely and Crawford Hall. The soil was in hard clumps that were dark gray-brown. |
| Naming Notes | I don't have a particular reason for naming this phage TeddyBear. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Sep 16, 2016 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 3701 |
| Genome length (bp) | 41555 |
| Character of genome ends | Circularly Permuted |
| GC Content | 63.4% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | EA |
| Subcluster | EA1 |
| Cluster Life Cycle | Lytic |
| Lysogeny Notes | Growth of mesas and lysogeny efficiency using phage-seeded plates was attempted with this phage by Audrey Jonas. This phage does not make a lysogen. |
| Other Cluster Members |
|
| Annotating Institution | University of Pittsburgh |
| Annotation Status | In GenBank |
| Plaque Notes | Plaques have different morphologies. The plaques are typically slightly turbid and about 1 mm in diameter, clear and about 2 mm in diameter, or clear and about 4 mm in diameter. If they are allowed to grow up for more than a day, a large halo will form around the plaques. The halos can be as large as 1.75 cm in diameter, if left to grow up for a several days. |
| Morphotype | Siphoviridae |
| Number of Genes | 62 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MH045564 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
5 |
| Pitt Freezer Box Grid# |
B8 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| Final DNAMaster File | Download |
| GenBank File for Phamerator | Download |
