| Detailed Information for Phage WildNOut |
| Discovery Information |
| Isolation Host | Microbacterium foliorum NRRL B-24224 |
| Found By | Mary Harshberger & Emily Tibble |
| Year Found | 2019 |
| Location Found | Kenosha, WI United States |
| Finding Institution | Carthage College |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Unknown |
| Isolation Temperature | 77°C |
| GPS Coordinates | 42.644 N, 87.8523 W Map |
| Discovery Notes | I found this soil in a grassy area at the University of Wisconsin - Parkside. It was a dry and sunny day. |
| Naming Notes | We decided to call our phage WildNOut because our phage was easily found in a direct isolation but we've had lots of trouble with dilutions and being able to see our plaques and consistent data. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 24, 2020 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 2098 |
| Genome length (bp) | 41796 |
| Character of genome ends | Circularly Permuted |
| GC Content | 63.4% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | EA |
| Subcluster | EA1 |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | Carthage College |
| Annotation Status | In GenBank |
| Plaque Notes | The plaques have always been large and temperate. There are some smaller plaques hidden under the larger ones but overall a temperate population. |
| Number of Genes | 63 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MW435859 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
105 |
| Pitt Freezer Box Grid# |
B10 |
| Available Files |
| GenBank File for Phamerator | Download |