| Detailed Information for Phage Winzigespinne |
| Discovery Information |
| Isolation Host | Microbacterium foliorum NRRL B-24224 |
| Found By | Adam Saal |
| Year Found | 2019 |
| Location Found | Ottawa, Canada |
| Finding Institution | University of Ottawa |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Unknown |
| Isolation Temperature | 15°C |
| GPS Coordinates | 45.4242 N, 75.6829 W Map |
| Discovery Notes | Phage environmental sample collected from a local garden. Unknown if garden was fertilized.
Soil was soft and airy
Soil was dark in colour |
| Naming Notes | Winzigespinne is roughly translated "Tiny Spider" to German. The inspiration comes from a reference mentioned in Stephanie Strathdee's novel "The Perfect Predator". It references the nursery rhyme "There was an old lady who swallowed a fly". The old lady swallows a spider to catch the fly. Strathdee compares the fly to an infectious bacteria and the phage the spider to catch that fly. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 4, 2020 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 1592 |
| Genome length (bp) | 41556 |
| Character of genome ends | Circularly Permuted |
| GC Content | 63.4% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | EA |
| Subcluster | EA1 |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | University of Ottawa |
| Annotation Status | In GenBank |
| Plaque Notes | Plaques are partialy turbid. Small to medium in size. |
| Morphotype | Siphoviridae |
| Number of Genes | 62 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MT986027 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
106 |
| Pitt Freezer Box Grid# |
E6 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| Virtual Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
