| Detailed Information for Phage YuuY |
| Discovery Information |
| Isolation Host | Microbacterium foliorum NRRL B-24224 |
| Found By | Uylae Kim |
| Year Found | 2019 |
| Location Found | Riverside, CA USA |
| Finding Institution | La Sierra University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 22°C |
| GPS Coordinates | 33.90861 N, 117.495 W Map |
| Discovery Notes | The soil was wet, possessing a mud-like texture as it was obtained from the side of a stream. The sample was initially a dark brown coloration with few light brown spots. Sample contained biodegradable plant organisms such as roots, flowers, and duck feces; the composition of the saturated soil consisted of sand and organic matter. Soil was retrieved from the surface of the ground and down about 2-3 inches below surface level. The time of day was 3:40 p.m. on January 8, 2019. |
| Naming Notes | My name is UY. SEA-PHAGES has taught me, as well as my peers, what it means to fail or to mess up in science. Even if a procedure was performed correctly, there were times when the experiment still did not work. It was discouraging. But the lesson I learned is that sometimes, to get to who and where I am today ("uY"), it required me to go backwards first ("Yu") in order to fix and perfect previous steps. My mistakes were no longer setbacks, but opportunities to make me a better scientist. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | May 23, 2019 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 2327 |
| Genome length (bp) | 40996 |
| Character of genome ends | Circularly Permuted |
| GC Content | 63.2% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | EA |
| Subcluster | EA10 |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | La Sierra University |
| Annotation Status | In GenBank |
| Plaque Notes | The plaques are clear, but surrounded by a dark halo ring. This indicates the presence of a lysogenic phage colony. The diameter of the clear inner circle alone is 2 mm, and the combined diameter of the circle with the outer ring is around 4 mm. |
| Morphotype | Siphoviridae |
| Number of Genes | 63 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MN284901 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
93 |
| Pitt Freezer Box Grid# |
A5 |
| Available Files |
| Plaque Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
