| Detailed Information for Phage Apiary |
| Discovery Information |
| Isolation Host | Rhodococcus equi NRRL B-16538 |
| Found By | Sierra Swieringa |
| Year Found | 2022 |
| Location Found | Grand Rapids, MI United States |
| Finding Institution | Calvin University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 42.933934 N, 85.591096 W Map |
| Discovery Notes | The soil sample was collected in the Calvin Campus Vegetable Garden by a squash plant and from deep, dark, rich soil. |
| Naming Notes | An apiary is a collection of beehives, beehives have honeycombs, and honeycombs are made up of hexagonal shapes. The name Apiary came about because phage capsids are hexagonal in shape also. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Mar 8, 2023 |
| Sequencing Facility | NC State Genomic Sciences Laboratory |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 4019 |
| Genome length (bp) | 67424 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | AGCCGCGTAC |
| GC Content | 67.4% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | CR |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | Calvin University |
| Annotation Status | In GenBank |
| Plaque Notes | Small pen point size, very clear, fairly round with slight variations in size, the average plaque size was 1mm, edges were mostly smooth with a few ridges on certain plaques |
| Morphotype | Siphoviridae |
| Number of Genes | 95 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | OR434026 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
162 |
| Pitt Freezer Box Grid# |
D12 |
| Available Files |
| GenBank File for Phamerator | Download |