Rhodococcus phage Apiary
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Detailed Information for Phage Apiary
Discovery Information
Isolation HostRhodococcus equi NRRL B-16538
Found BySierra Swieringa
Year Found2022
Location FoundGrand Rapids, MI United States
Finding InstitutionCalvin University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature30°C
GPS Coordinates42.933934 N, 85.591096 W Map
Discovery NotesThe soil sample was collected in the Calvin Campus Vegetable Garden by a squash plant and from deep, dark, rich soil.
Naming NotesAn apiary is a collection of beehives, beehives have honeycombs, and honeycombs are made up of hexagonal shapes. The name Apiary came about because phage capsids are hexagonal in shape also.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedMar 8, 2023
Sequencing FacilityNC State Genomic Sciences Laboratory
Shotgun Sequencing MethodIllumina
Approximate Shotgun Coverage4019
Genome length (bp)67424
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceAGCCGCGTAC
GC Content67.4%
Fasta file available?Yes: Download fasta file
Characterization
ClusterCR
Subcluster--
Cluster Life CycleLytic
Other Cluster Members
Annotating InstitutionCalvin University
Annotation StatusIn GenBank
Plaque NotesSmall pen point size, very clear, fairly round with slight variations in size, the average plaque size was 1mm, edges were mostly smooth with a few ridges on certain plaques
MorphotypeSiphoviridae
Number of Genes95
Number of tRNAs0
Number of tmRNAs0
Has been Phamerated?Yes
Gene List

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Apiary_1
(terminase small subunit)

Apiary_2

Apiary_3

Apiary_4

Apiary_5

Apiary_6

Apiary_7

Apiary_8

Apiary_9

Apiary_10

Apiary_11

Apiary_12

Apiary_13

Apiary_14

Apiary_15

Apiary_16

Apiary_17

Apiary_18
(ThyX-like thymidylate synthase)

Apiary_19

Apiary_20
(lysin B)

Apiary_21
(MazG-like nucleotide pyrophosphohydrolase)

Apiary_22

Apiary_23
(minor tail protein)

Apiary_24

Apiary_25

Apiary_26

Apiary_27
(HNH endonuclease)

Apiary_28
(terminase large subunit)

Apiary_29
(portal protein)

Apiary_30

Apiary_31
(scaffolding protein)

Apiary_32
(capsid decoration protein)

Apiary_33
(major capsid protein)

Apiary_34

Apiary_35
(head-to-tail adaptor)

Apiary_36
(head-to-tail stopper)

Apiary_37

Apiary_38
(tail terminator)

Apiary_39
(major tail protein)

Apiary_40

Apiary_41
(tail assembly chaperone)

Apiary_42
(tail assembly chaperone)

Apiary_43
(tape measure protein)

Apiary_44
(minor tail protein)

Apiary_45
(minor tail protein)

Apiary_46
(minor tail protein)

Apiary_47

Apiary_48
(minor tail protein)

Apiary_49

Apiary_50
(lysin A)

Apiary_51
(holin)

Apiary_52

Apiary_53
(DNA primase/polymerase)

Apiary_54

Apiary_55
(WhiB family transcription factor)

Apiary_56

Apiary_57
(helix-turn-helix DNA-binding domain protein)

Apiary_58
(RuvC-like resolvase)

Apiary_59
(DnaE-like DNA polymerase III alpha)

Apiary_60

Apiary_61

Apiary_62

Apiary_63

Apiary_64
(ASCE ATPase)

Apiary_65

Apiary_66

Apiary_67
(exonuclease)

Apiary_68

Apiary_69

Apiary_70

Apiary_71

Apiary_72
(DNA helicase)

Apiary_73

Apiary_74

Apiary_75
(RtcB-like RNA ligase)

Apiary_76

Apiary_77

Apiary_78
(DNA binding protein)

Apiary_79

Apiary_80

Apiary_81

Apiary_82

Apiary_83

Apiary_84

Apiary_85

Apiary_86

Apiary_87

Apiary_88

Apiary_89

Apiary_90

Apiary_91

Apiary_92

Apiary_93

Apiary_94

Apiary_95

Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionOR434026
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 162
Pitt Freezer Box Grid# D12
Available Files
GenBank File for PhameratorDownload