Gordonia phage Patio
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Detailed Information for Phage Patio
Discovery Information
Isolation HostGordonia terrae 3612
Former namesTeaPea
Found BySarah Kuchan
Year Found2016
Location FoundPittsburgh, PA USA
Finding InstitutionUniversity of Pittsburgh
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates40.442840, -79.956205 Map
Discovery Notes-at time of soil collection it was a dry overcast day and was 26 F
-The soil was frozen
-the weather conditions over the last 24 hours were around 10 F and windy + overcast
-the soil was collected less than an hour before class (this is new)
-the soil had fertilizer pellets (blue dots) on it
Naming NotesOriginally, I wanted to name him TP because I found him on the patio of one of our residence halls- Towers Patio. However, when I read the naming rules I saw the names could not be only 2 letters, so I extended it to TeePee (I still get the same effect). Then, that name was already taken. To combat this catastrophe, I changed it to TeaPea. Like the tea you drink + peas you eat. Very clever.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJun 30, 2016
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage3811
Genome length (bp)66251
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceCGCCGCGTAC
GC Content65.6%
Sequencing Notes22% of reads call 10 G's instead of consensus 9 between 65907 - 65917 bp
Fasta file available?Yes: Download fasta file
Characterization
ClusterCR
SubclusterCR3
Cluster Life CycleLytic
Lysogeny Notes-temperate phage
Other Cluster Members
Annotating InstitutionUnknown or unassigned
Annotation StatusIn GenBank
Plaque Notes-large plaques
-halos in all of them
-range from 1 mm- 3mm.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

Click to View

Patio_1
(HNH endonuclease)

Patio_2
(terminase small subunit)

Patio_3

Patio_4

Patio_5

Patio_6

Patio_7

Patio_8

Patio_9

Patio_10

Patio_11

Patio_12
(HicA-like toxin)

Patio_13

Patio_14

Patio_15

Patio_16

Patio_17

Patio_18

Patio_19

Patio_20

Patio_21
(lysin B)

Patio_22

Patio_23
(major tail protein)

Patio_24

Patio_25

Patio_26
(HNH endonuclease)

Patio_27
(terminase large subunit)

Patio_28
(portal protein)

Patio_29
(capsid maturation protease)

Patio_30
(scaffolding protein)

Patio_31
(capsid decoration protein)

Patio_32
(major capsid protein)

Patio_33
(head-to-tail connector protein)

Patio_34
(head-to-tail connector protein)

Patio_35
(head-to-tail connector protein)

Patio_36
(head-to-tail connector protein)

Patio_37
(head-to-tail connector protein)

Patio_38
(major tail protein)

Patio_39

Patio_40
(tail assembly chaperone)

Patio_41
(tail assembly chaperone)

Patio_42
(tapemeasure protein)

Patio_43
(minor tail protein)

Patio_44
(minor tail protein)

Patio_45
(minor tail protein)

Patio_46
(minor tail protein)

Patio_47

Patio_48

Patio_49

Patio_50

Patio_51
(lysin A)

Patio_52
(lysin A)

Patio_53
(holin)

Patio_54

Patio_55

Patio_56
(DNA primase/polymerase)

Patio_57

Patio_58
(WhiB family transcription factor)

Patio_59

Patio_60
(HTH DNA binding domain protein)

Patio_61
(resolvase)

Patio_62
(DNA polymerase III alpha subunit)

Patio_63

Patio_64

Patio_65

Patio_66

Patio_67
(AAA-ATPase)

Patio_68
(nuclease)

Patio_69

Patio_70

Patio_71

Patio_72

Patio_73

Patio_74
(helicase)

Patio_75

Patio_76

Patio_77

Patio_78

Patio_79

Patio_80

Patio_81

Patio_82

Patio_83

Patio_84

Patio_85

Patio_86

Patio_87

Patio_88

Patio_89

Patio_90

Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMF919542
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 8.0x10^9
Date of SEA Lysate Titering Feb 29, 2016
Pitt Freezer Box# 3
Pitt Freezer Box Grid# F7
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload