The Actinobacteriophage Database

Rhodococcus phage Polyyuki

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Detailed Information for Phage Polyyuki
Discovery Information
Isolation Host	Rhodococcus equi NRRL B-16538
Found By	Matt Minasian
Year Found	2022
Location Found	Grand Rapids, MI United States
Finding Institution	Calvin University
Program	Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?	Unknown
Isolation Temperature	Not entered
GPS Coordinates	Unavailable
Discovery Notes	Sample was taken up a kept-up garden area with plants. Dirt was dry. Isolated it after 3 tries since the first 2 tries seemed to have different plaques.
Naming Notes	Named after 2 of my pets. I have a dog named Polly and a cat named Yuki, so the name is a combination of both of their names.
Sequencing Information
Sequencing Complete?	Yes
Date Sequencing Completed	Mar 14, 2023
Sequencing Facility	Pittsburgh Bacteriophage Institute
Shotgun Sequencing Method	Illumina Sequencing
Approximate Shotgun Coverage	3660
Genome length (bp)	67447
Character of genome ends	3' Sticky Overhang
Overhang Length	10 bases
Overhang Sequence	AGCCGCGTAC
GC Content	67.3%
Fasta file available?	Available upon entering GPS coordinates
Characterization
Cluster	CR
Subcluster	--
Cluster Life Cycle	Lytic
Other Cluster Members	Click to View Apiary (None) Braxoaddie (None) GuyFagieri (None) MacGully (None) Maselop (None) Polyyuki (None) Dalilpop (CR1) Flapper (CR1) GRU1 (CR1) GTE5 (CR1) Turuncu (CR1) Arti (CR2) Bonum (CR2) Buggaboo (CR2) Commandaria (CR2) Emianna (CR2) Foxboro (CR2) GrootJr (CR2) GTE8 (CR2) Jifall16 (CR2) Kabluna (CR2) KidneyBean (CR2) Kurt (CR2) MerCougar (CR2) NatB6 (CR2) NosilaM (CR2) NovumRegina (CR2) Outis (CR2) Phomeo (CR2) StarStruck (CR2) SuperSulley (CR2) Tracker (CR2) Wheezy (CR2) Ennea (CR3) Float294 (CR3) Lollipop1437 (CR3) Patio (CR3) Skysand (CR3) BiPauneto (CR4) IDyn (CR4) Marietta (CR4) NadineRae (CR4) Sukkupi (CR4) WhoseManz (CR4) Yndexa (CR4) Fury (CR5) Pleakley (CR5)
Annotating Institution	Calvin University
Annotation Status	Being Annotated (Expected completion by 9/14/2023)
Plaque Notes	Plaques are small, circular, about 1 mm in diameter. They seem to have defined edges and are clear.
Morphotype	Siphoviridae
Has been Phamerated?	Yes
Gene List	Click to View Polyyuki_Draft_1 Polyyuki_Draft_2 Polyyuki_Draft_3 Polyyuki_Draft_4 Polyyuki_Draft_5 Polyyuki_Draft_6 Polyyuki_Draft_7 Polyyuki_Draft_8 Polyyuki_Draft_9 Polyyuki_Draft_10 Polyyuki_Draft_11 Polyyuki_Draft_12 Polyyuki_Draft_13 Polyyuki_Draft_14 Polyyuki_Draft_15 Polyyuki_Draft_16 Polyyuki_Draft_17 Polyyuki_Draft_18 Polyyuki_Draft_19 Polyyuki_Draft_20 Polyyuki_Draft_21 Polyyuki_Draft_22 Polyyuki_Draft_23 Polyyuki_Draft_24 Polyyuki_Draft_25 Polyyuki_Draft_26 Polyyuki_Draft_27 Polyyuki_Draft_28 Polyyuki_Draft_29 Polyyuki_Draft_30 Polyyuki_Draft_31 Polyyuki_Draft_32 Polyyuki_Draft_33 Polyyuki_Draft_34 Polyyuki_Draft_35 Polyyuki_Draft_36 Polyyuki_Draft_37 Polyyuki_Draft_38 Polyyuki_Draft_39 Polyyuki_Draft_40 Polyyuki_Draft_41 Polyyuki_Draft_42 Polyyuki_Draft_43 Polyyuki_Draft_44 Polyyuki_Draft_45 Polyyuki_Draft_46 Polyyuki_Draft_47 Polyyuki_Draft_48 Polyyuki_Draft_49 Polyyuki_Draft_50 Polyyuki_Draft_51 Polyyuki_Draft_52 Polyyuki_Draft_53 Polyyuki_Draft_54 Polyyuki_Draft_55 Polyyuki_Draft_56 Polyyuki_Draft_57 Polyyuki_Draft_58 Polyyuki_Draft_59 Polyyuki_Draft_60 Polyyuki_Draft_61 Polyyuki_Draft_62 Polyyuki_Draft_63 Polyyuki_Draft_64 Polyyuki_Draft_65 Polyyuki_Draft_66 Polyyuki_Draft_67 Polyyuki_Draft_68 Polyyuki_Draft_69 Polyyuki_Draft_70 Polyyuki_Draft_71 Polyyuki_Draft_72 Polyyuki_Draft_73 Polyyuki_Draft_74 Polyyuki_Draft_75 Polyyuki_Draft_76 Polyyuki_Draft_77 Polyyuki_Draft_78 Polyyuki_Draft_79 Polyyuki_Draft_80 Polyyuki_Draft_81 Polyyuki_Draft_82 Polyyuki_Draft_83 Polyyuki_Draft_84 Polyyuki_Draft_85 Polyyuki_Draft_86 Polyyuki_Draft_87 Polyyuki_Draft_88 Polyyuki_Draft_89 Polyyuki_Draft_90 Polyyuki_Draft_91 Polyyuki_Draft_92 Polyyuki_Draft_93 Polyyuki_Draft_94
Publication Info
Uploaded to GenBank?	No
GenBank Accession	None yet
Refseq Number	None yet
Archiving Info
Archiving status	Archived
Pitt Freezer Box#	162
Pitt Freezer Box Grid#	F4

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