Gordonia phage StarStruck
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Detailed Information for Phage StarStruck
Discovery Information
Isolation HostGordonia terrae 3612
Found ByEleanor Carrolton and Julia Coombs
Year Found2021
Location FoundOrono, ME United States of America
Finding InstitutionUniversity of Maine, Honors College
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature21°C
GPS Coordinates44.896455 N, 68.66614 W Map
Discovery NotesFound in the garden bed of the Soil and Water Laboratory at Maine. The soil was soft and well aerated.
Naming NotesWe were thinking about naming our phage after a constellation. While exploring available names related to stars we saw that just StarStruck was available. We liked this name and thought it would be fun to abreviate it to St^2 on tubes.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 18, 2022
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage609
Genome length (bp)68128
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceCGCCGCGTAC
GC Content65.4%
Fasta file available?Yes: Download fasta file
Characterization
ClusterCR
SubclusterCR2
Cluster Life CycleLytic
Other Cluster Members
Annotating InstitutionUniversity of Maine, Honors College
Annotation StatusIn GenBank
Plaque NotesThe plaque edges of this phage are turbid. It is incredibly aggressive and must be plated out very far to be observed. Otherwise, it easily lyses the g.terrae. When there is high competition the plaque morphology ranges from 0.1cm to 0.4cm in diameter. When there is less competition it adopts the larger morphology.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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StarStruck_1
(terminase, small subunit)

StarStruck_2

StarStruck_3

StarStruck_4

StarStruck_5

StarStruck_6

StarStruck_7
(toxin in toxin/antitoxin system, HicA-like)

StarStruck_8

StarStruck_9

StarStruck_10

StarStruck_11

StarStruck_12

StarStruck_13

StarStruck_14

StarStruck_15

StarStruck_16

StarStruck_17

StarStruck_18

StarStruck_19
(lysin B)

StarStruck_20

StarStruck_21
(hydrolase)

StarStruck_22

StarStruck_23

StarStruck_24
(HNH endonuclease)

StarStruck_25
(terminase, large subunit)

StarStruck_26
(portal protein)

StarStruck_27

StarStruck_28
(scaffolding protein)

StarStruck_29
(capsid decoration protein, LamD-like)

StarStruck_30
(major capsid protein)

StarStruck_31

StarStruck_32
(head-to-tail adaptor)

StarStruck_33
(head-to-tail stopper)

StarStruck_34

StarStruck_35
(tail terminator)

StarStruck_36
(major tail protein)

StarStruck_37

StarStruck_38
(tail assembly chaperone)

StarStruck_39
(tail assembly chaperone)

StarStruck_40
(tape measure protein)

StarStruck_41
(minor tail protein)

StarStruck_42
(minor tail protein)

StarStruck_43
(minor tail protein)

StarStruck_44
(minor tail protein)

StarStruck_45

StarStruck_46
(minor tail protein)

StarStruck_47

StarStruck_48

StarStruck_49
(lysin A, protease C39 domain)

StarStruck_50
(lysin A, glycosyl hydrolase domain)

StarStruck_51
(holin)

StarStruck_52

StarStruck_53

StarStruck_54
(DNA primase/polymerase/helicase)

StarStruck_55

StarStruck_56
(WhiB family transcription factor)

StarStruck_57

StarStruck_58
(helix-turn-helix DNA binding domain, MerR-like)

StarStruck_59
(RuvC-like resolvase)

StarStruck_60

StarStruck_61
(DnaE-like DNA polymerase III (alpha))

StarStruck_62

StarStruck_63

StarStruck_64
(thio)

StarStruck_65

StarStruck_66
(RecA-like DNA recombinase)

StarStruck_67
(Cas4 family exonuclease)

StarStruck_68

StarStruck_69

StarStruck_70

StarStruck_71

StarStruck_72

StarStruck_73
(DNA helicase)

StarStruck_74
(WhiB family transcription factor)

StarStruck_75
(thioredoxin)

StarStruck_76

StarStruck_77

StarStruck_78

StarStruck_79

StarStruck_80

StarStruck_81

StarStruck_82

StarStruck_83

StarStruck_84

StarStruck_85

StarStruck_86

StarStruck_87

StarStruck_88

StarStruck_89

StarStruck_90

StarStruck_91

StarStruck_92

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionON456333
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 142
Pitt Freezer Box Grid# E1
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload