Gordonia phage Archis
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Detailed Information for Phage Archis
Discovery Information
Isolation HostGordonia terrae 3612
Found ByArchisha Ghosh
Year Found2019
Location FoundPittsburgh, PA USA
Finding InstitutionUniversity of Pittsburgh
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation Temperature30°C
GPS Coordinates40.442222 N, 80.01 W Map
Discovery NotesThere was a 730 ft elevation and there was vegetation around. However, the soil was also clumped together in some places, as if some places were wet. The temperature was about 75 degrees and the soil was taken from just barely below the surface.
Naming NotesThe phage name is simply the first six letters of my first name.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 5, 2020
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage1222
Genome length (bp)49000
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceTCTCCGGTGA
GC Content67.3%
Fasta file available?Yes: Download fasta file
Characterization
ClusterCV
Subcluster--
Cluster Life CycleTemperate
Other Cluster Members
Annotating InstitutionUniversity of Pittsburgh
Annotation StatusIn GenBank
Plaque NotesThe capsids are approximately 83 nm wide and the tails are about 442 nm long. The phage creates plaques that are turbid which means the phage is temperate.
MorphotypeSiphoviridae
Number of Genes82
Number of tRNAs0
Number of tmRNAs0
Has been Phamerated?Yes
Gene List

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Archis_1
(terminase small subunit)

Archis_2
(terminase large subunit)

Archis_3
(portal protein)

Archis_4
(capsid maturation protease)

Archis_5
(scaffolding protein)

Archis_6
(major capsid protein)

Archis_7

Archis_8
(head-to-tail adaptor)

Archis_9

Archis_10
(head-to-tail stopper)

Archis_11

Archis_12

Archis_13

Archis_14
(tail terminator)

Archis_15
(major tail protein)

Archis_16
(tail assembly chaperone)

Archis_17
(tail assembly chaperone)

Archis_18
(tape measure protein)

Archis_19
(minor tail protein)

Archis_20
(minor tail protein)

Archis_21
(lysin A, L-Ala-D-Glu peptidase domain)

Archis_22
(lysin A, glycosyl hydrolase domain)

Archis_23
(holin)

Archis_24

Archis_25

Archis_26
(lysin B)

Archis_27

Archis_28
(minor tail protein)

Archis_29
(minor tail protein)

Archis_30

Archis_31
(minor tail protein)

Archis_32

Archis_33

Archis_34

Archis_35

Archis_36

Archis_37

Archis_38
(RelB-like antitoxin)

Archis_39
(RelE-like toxin)

Archis_40

Archis_41
(helix-turn-helix DNA binding domain protein)

Archis_42

Archis_43
(tyrosine integrase)

Archis_44

Archis_45
(immunity repressor)

Archis_46

Archis_47

Archis_48

Archis_49

Archis_50

Archis_51

Archis_52

Archis_53

Archis_54
(helix-turn-helix DNA binding domain protein)

Archis_55

Archis_56

Archis_57

Archis_58

Archis_59

Archis_60
(RecE-like exonuclease)

Archis_61
(RecT-like ssDNA binding protein)

Archis_62

Archis_63

Archis_64

Archis_65

Archis_66

Archis_67

Archis_68

Archis_69
(helix-turn-helix DNA binding domain protein)

Archis_70
(methyltransferase)

Archis_71

Archis_72
(helix-turn-helix DNA binding domain protein)

Archis_73

Archis_74

Archis_75

Archis_76

Archis_77
(RuvC-like resolvase)

Archis_78

Archis_79
(helix-turn-helix DNA binding domain protein)

Archis_80

Archis_81
(G-I-Y Y-I-G endonuclease)

Archis_82
(HNH endonuclease)

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMT723942
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 1.17 x 10^11
Pitt Freezer Box# 113
Pitt Freezer Box Grid# E9
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload