Gordonia phage Walrus
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Detailed Information for Phage Walrus
Discovery Information
Isolation HostGordonia terrae 3612
Found ByMatthew Montgomery
Year Found2016
Location FoundPittsburgh, PA USA
Finding InstitutionUniversity of Pittsburgh
ProgramPhage Hunters Integrating Research and Education
From enriched soil sample?No
Isolation Temperature30°C
GPS Coordinates40.442567 N, 79.957276 W Map
Discovery NotesThis phage was a contaminant in a BetterKatz lysate. I isolated it by plating the contaminated lysate on the Betterkatz lysogen (so only the contaminant can infect). From there I purified and amplified using standard protocols.
Naming NotesWalruses are interesting animals.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 20, 2017
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage551
Genome length (bp)52024
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceTGGCCGGTGA
GC Content67.3%
Fasta file available?Yes: Download fasta file
Characterization
ClusterCV
Subcluster--
Cluster Life CycleTemperate
Lysogeny NotesEmperor is able to form a G. terrae 3612 lysogen.
Other Cluster Members
Annotation StatusIn GenBank
Plaque NotesPlaques are small and turbid (may form lysogen).
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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Walrus_1
(terminase, small subunit)

Walrus_2
(terminase, large subunit)

Walrus_3
(portal protein)

Walrus_4
(capsid maturation protease)

Walrus_5
(scaffolding protein)

Walrus_6
(major capsid protein)

Walrus_7

Walrus_8
(head-to-tail adaptor)

Walrus_9
(head-to-tail stopper)

Walrus_10

Walrus_11

Walrus_12

Walrus_13

Walrus_14
(tail terminator)

Walrus_15
(major tail protein)

Walrus_16
(tail assembly chaperone)

Walrus_17
(tail assembly chaperone)

Walrus_18
(tape measure protein)

Walrus_19
(minor tail protein)

Walrus_20
(minor tail protein)

Walrus_21
(lysin A, L-ala, D-glu peptidase domain)

Walrus_22

Walrus_23
(lysin A, glycosyl hydrolase domain)

Walrus_24
(membrane protein)

Walrus_25
(membrane protein)

Walrus_26
(membrane protein)

Walrus_27
(lysin B)

Walrus_28

Walrus_29
(minor tail protein)

Walrus_30
(minor tail protein)

Walrus_31

Walrus_32
(minor tail protein)

Walrus_33

Walrus_34

Walrus_35
(minor tail protein)

Walrus_36

Walrus_37

Walrus_38
(helix-turn helix DNA-binding domain protein)

Walrus_39
(membrane protein)

Walrus_40
(integrase (Y-int))

Walrus_41
(immunity repressor)

Walrus_42
(excise)

Walrus_43

Walrus_44

Walrus_45

Walrus_46

Walrus_47

Walrus_48

Walrus_49

Walrus_50

Walrus_51

Walrus_52

Walrus_53

Walrus_54

Walrus_55
(DnaQ-like exonuclease)

Walrus_56
(RecE exonuclease)

Walrus_57
(RecT-like ssDNA binding protein)

Walrus_58

Walrus_59

Walrus_60

Walrus_61

Walrus_62

Walrus_63
(nucleotidyltransferase)

Walrus_64

Walrus_65

Walrus_66
(DNA methylase)

Walrus_67

Walrus_68
(HNH endonuclease)

Walrus_69
(helix-turn-helix DNA binding protein)

Walrus_70

Walrus_71

Walrus_72

Walrus_73
(RuvC-like resolvase)

Walrus_74

Walrus_75

Walrus_76

Walrus_77
(helix-turn-helix DNA binding protein)

Walrus_78

Walrus_79

Walrus_80

Walrus_81

Walrus_82

Walrus_83

Walrus_84
(HNH endonuclease)

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMK501729
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 6
Pitt Freezer Box Grid# E8
Available Files
Final DNAMaster FileDownload
GenBank File for PhameratorDownload