| Detailed Information for Phage Archis |
| Discovery Information |
| Isolation Host | Gordonia terrae 3612 |
| Found By | Archisha Ghosh |
| Year Found | 2019 |
| Location Found | Pittsburgh, PA USA |
| Finding Institution | University of Pittsburgh |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 30°C |
| GPS Coordinates | 40.442222 N, 80.01 W Map |
| Discovery Notes | There was a 730 ft elevation and there was vegetation around. However, the soil was also clumped together in some places, as if some places were wet. The temperature was about 75 degrees and the soil was taken from just barely below the surface. |
| Naming Notes | The phage name is simply the first six letters of my first name. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 5, 2020 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 1222 |
| Genome length (bp) | 49000 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | TCTCCGGTGA |
| GC Content | 67.3% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | CV |
| Subcluster | -- |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | University of Pittsburgh |
| Annotation Status | In GenBank |
| Plaque Notes | The capsids are approximately 83 nm wide and the tails are about 442 nm long. The phage creates plaques that are turbid which means the phage is temperate. |
| Morphotype | Siphoviridae |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MT723942 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
1.17 x 10^11 |
| Pitt Freezer Box# |
113 |
| Pitt Freezer Box Grid# |
E9 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
