Gordonia phage PhrostedPhlake
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Detailed Information for Phage PhrostedPhlake
Discovery Information
Isolation HostGordonia terrae 3612
Found ByDeborah Lee
Year Found2017
Location FoundPittsburgh, PA USA
Finding InstitutionUniversity of Pittsburgh
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation TemperatureNot entered
GPS Coordinates40.443558 N, 79.960855 W Map
Discovery NotesI collected this sample from a garden bed outside of Scaife Hall at the University of Pittsburgh. The soil was moist, soft, and easy to collect. The weather conditions at the time of collection were 12°C, overcast, and not windy at all. The weather conditions in the last 24 hours were light showers with a high of 4°C and a low of -3°C. Sample was transported for 30 minutes prior to storage and stored for 1 hour in a desk drawer at 70°C.
Naming NotesThis phage is named after Kellogg's Frosted Flakes cereal.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedMay 17, 2017
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage1954
Genome length (bp)52042
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceTCTCCGGTGA
GC Content67.4%
Fasta file available?Yes: Download fasta file
Characterization
ClusterCV
Subcluster--
Cluster Life CycleTemperate
Other Cluster Members
Annotation StatusIn GenBank
Plaque NotesThe plaques are all tiny, clear, and circular, and the morphology is very consistent. Almost all of the plaques have a diameter of 1mm.
Has been Phamerated?Yes
Gene List

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PhrostedPhlake_1
(terminase, small subunit)

PhrostedPhlake_2
(terminase, large subunit)

PhrostedPhlake_3
(portal protein)

PhrostedPhlake_4
(capsid maturation protease)

PhrostedPhlake_5
(scaffolding protein)

PhrostedPhlake_6
(major capsid protein)

PhrostedPhlake_7

PhrostedPhlake_8
(head-to-tail adaptor)

PhrostedPhlake_9
(head-to-tail stopper)

PhrostedPhlake_10

PhrostedPhlake_11

PhrostedPhlake_12

PhrostedPhlake_13

PhrostedPhlake_14
(tail terminator)

PhrostedPhlake_15
(major tail protein)

PhrostedPhlake_16
(tail assembly chaperone)

PhrostedPhlake_17
(tail assembly chaperone)

PhrostedPhlake_18
(tape measure protein)

PhrostedPhlake_19
(minor tail protein)

PhrostedPhlake_20
(minor tail protein)

PhrostedPhlake_21
(lysin A, L-Ala-D-Glu peptidase domain)

PhrostedPhlake_22

PhrostedPhlake_23
(lysin A, glycosyl hydrolase domain)

PhrostedPhlake_24
(holin)

PhrostedPhlake_25
(membrane protein)

PhrostedPhlake_26
(membrane protein)

PhrostedPhlake_27
(lysin B)

PhrostedPhlake_28

PhrostedPhlake_29
(minor tail protein)

PhrostedPhlake_30
(minor tail protein)

PhrostedPhlake_31

PhrostedPhlake_32
(minor tail protein)

PhrostedPhlake_33

PhrostedPhlake_34
(membrane protein)

PhrostedPhlake_35

PhrostedPhlake_36

PhrostedPhlake_37

PhrostedPhlake_38
(ribbon-helix-helix DNA binding domain)

PhrostedPhlake_39
(toxin in toxin/antitoxin system, BrnT-like)

PhrostedPhlake_40
(membrane protein)

PhrostedPhlake_41
(helix-turn-helix DNA binding domain)

PhrostedPhlake_42

PhrostedPhlake_43
(integrase, (Y-int))

PhrostedPhlake_44
(immunity repressor)

PhrostedPhlake_45
(helix-turn-helix DNA binding domain)

PhrostedPhlake_46
(membrane protein)

PhrostedPhlake_47

PhrostedPhlake_48

PhrostedPhlake_49

PhrostedPhlake_50

PhrostedPhlake_51

PhrostedPhlake_52

PhrostedPhlake_53

PhrostedPhlake_54

PhrostedPhlake_55

PhrostedPhlake_56

PhrostedPhlake_57

PhrostedPhlake_58

PhrostedPhlake_59
(exonuclease)

PhrostedPhlake_60
(RecT-like ssDNA binding protein)

PhrostedPhlake_61

PhrostedPhlake_62

PhrostedPhlake_63

PhrostedPhlake_64

PhrostedPhlake_65

PhrostedPhlake_66
(membrane protein)

PhrostedPhlake_67
(ASC-1 transcription coactivator)

PhrostedPhlake_68

PhrostedPhlake_69

PhrostedPhlake_70
(DNA methylase)

PhrostedPhlake_71

PhrostedPhlake_72
(membrane protein)

PhrostedPhlake_73

PhrostedPhlake_74
(HNH endonuclease)

PhrostedPhlake_75

PhrostedPhlake_76
(HNH endonuclease)

PhrostedPhlake_77

PhrostedPhlake_78

PhrostedPhlake_79

PhrostedPhlake_80

PhrostedPhlake_81
(RuvC-like resolvase)

PhrostedPhlake_82

PhrostedPhlake_83
(helix-turn-helix DNA binding domain)

PhrostedPhlake_84

PhrostedPhlake_85

PhrostedPhlake_86

PhrostedPhlake_87

PhrostedPhlake_88
(HNH endonuclease)

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMK801724
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 5E10
Pitt Freezer Box# 56
Pitt Freezer Box Grid# B7
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload