| Detailed Information for Phage Azula |
| Discovery Information |
| Isolation Host | Gordonia terrae 3612 |
| Found By | Maya Albanowski and Rachel DeCesaris |
| Year Found | 2019 |
| Location Found | Pittsburgh, PA United States |
| Finding Institution | University of Pittsburgh |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 30°C |
| GPS Coordinates | 40.4504 N, 79.951 W Map |
| Discovery Notes | One day when walking to class, Maya collected dirt next to the sidewalk in North Oakland in Pittsburgh, PA. The soil was dry, grey, and sandy and was near to grass. The weather at time of collection had been hot and dry for several weeks. |
| Naming Notes | Azula is the sister of Prince Zuko in Avatar: The Last Airbender. On the show, she is a villainous firebender who is known for her ruthlessness. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 5, 2020 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 1285 |
| Genome length (bp) | 53622 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | TGGCCGGTGA |
| GC Content | 67.1% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | CV |
| Subcluster | -- |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | University of Pittsburgh |
| Annotation Status | In GenBank |
| Plaque Notes | This phage forms small plaques that are clear in the center with a halo around the edges. |
| Number of Genes | 86 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MT723935 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
113 |
| Pitt Freezer Box Grid# |
F6 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
