| Detailed Information for Phage Barco | |
| Discovery Information | |
| Isolation Host | Gordonia terrae 3612 |
| Found By | Kayla Albert |
| Year Found | 2016 |
| Location Found | Pittsburgh, PA USA |
| Finding Institution | University of Pittsburgh |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 40.447778 N, 79.959722 W Map |
| Discovery Notes | The phage was discovered in a garden outside of a Law Building. The soil sample the phage came from was collected during cool weather conditions (51 degrees Fahrenheit) and sunny. The weather conditions in the previous couple of days have been colder (high of 48 and low of 23 degrees Fahrenheit) and rainy. |
| Naming Notes | The name of this phage was inspired by the building it was found outside of, the Barco Law Building. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | May 3, 2016 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 2927 |
| Genome length (bp) | 48905 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | TCGCCGGTGA |
| GC Content | 67.3% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | CV |
| Subcluster | -- |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
Click to ViewArchis Azula Barco Begonia BlingBling Blino Blueberry CaptainKirk2 CarolAnn Delian Fairfaxidum Fenry Frokostdame Gambino Guacamole Hitter Jalammah JasperJr JuJu KappaFarmDelta Lysidious Malachai Melba Mellie MintFen MissRona Murp Obliviate PCoral7 Petra PhrostedPhlake Pipp PrincePatrick Samba Toast UmaThurman Utz Walrus William Wisp Wocket Zarbodnamra ZiggyZoo |
| Annotating Institution | Gettysburg College |
| Annotation Status | In GenBank |
| Plaque Notes | The plaque size ranges from 0.5mm to 2mm with the majority being on the larger size. The plaques have a halo shape with clear centers and cloudy outer rings (turbid) |
| Morphotype | Siphoviridae |
| Number of Genes | 77 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewBarco_1 Barco_2 Barco_3 Barco_4 Barco_5 Barco_6 Barco_7 Barco_8 Barco_9 Barco_10 Barco_11 Barco_12 Barco_13 Barco_14 Barco_15 Barco_16 Barco_17 Barco_18 Barco_19 Barco_20 Barco_21 Barco_22 Barco_23 Barco_24 Barco_25 Barco_26 Barco_27 Barco_28 Barco_29 Barco_30 Barco_31 Barco_32 Barco_33 Barco_34 Barco_35 Barco_36 Barco_37 Barco_38 Barco_39 Barco_40 Barco_41 Barco_42 Barco_43 Barco_44 Barco_45 Barco_46 Barco_47 Barco_48 Barco_49 Barco_50 Barco_51 Barco_52 Barco_53 Barco_54 Barco_55 Barco_56 Barco_57 Barco_58 Barco_59 Barco_60 Barco_61 Barco_62 Barco_63 Barco_64 Barco_65 Barco_66 Barco_67 Barco_68 Barco_69 Barco_70 Barco_71 Barco_72 Barco_73 Barco_74 Barco_75 Barco_76 Barco_77 |
| Submitted Minimal DNA Master File | Download |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MK501730 |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| SEA Lysate Titer | 1.46x10^11 pfu/mL |
| Date of SEA Lysate Titering | Feb 22, 2016 |
| Pitt Freezer Box# | 3 |
| Pitt Freezer Box Grid# | C1 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| Final DNAMaster File | Download |
| GenBank File for Phamerator | Download |
