Gordonia phage Barco
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| Detailed Information for Phage Barco | |
| Discovery Information | |
| Isolation Host | Gordonia terrae 3612 |
| Found By | Kayla Albert |
| Year Found | 2016 |
| Location Found | Pittsburgh, PA USA |
| Finding Institution | University of Pittsburgh |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 40.447778 N, 79.959722 W Map |
| Discovery Notes | The phage was discovered in a garden outside of a Law Building. The soil sample the phage came from was collected during cool weather conditions (51 degrees Fahrenheit) and sunny. The weather conditions in the previous couple of days have been colder (high of 48 and low of 23 degrees Fahrenheit) and rainy. |
| Naming Notes | The name of this phage was inspired by the building it was found outside of, the Barco Law Building. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | May 3, 2016 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 2927 |
| Genome length (bp) | 48905 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | TCGCCGGTGA |
| GC Content | 67.3% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | CV |
| Subcluster | -- |
| Cluster Life Cycle | Temperate |
| Other Cluster Members | |
| Annotating Institution | Gettysburg College |
| Annotation Status | In GenBank |
| Plaque Notes | The plaque size ranges from 0.5mm to 2mm with the majority being on the larger size. The plaques have a halo shape with clear centers and cloudy outer rings (turbid) |
| Morphotype | Siphoviridae |
| Number of Genes | 77 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List | |
| Submitted Minimal DNA Master File | Download |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MK501730 |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| SEA Lysate Titer | 1.46x10^11 pfu/mL |
| Date of SEA Lysate Titering | Feb 22, 2016 |
| Pitt Freezer Box# | 3 |
| Pitt Freezer Box Grid# | C1 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| Final DNAMaster File | Download |
| GenBank File for Phamerator | Download |
