Gordonia phage Barco
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Detailed Information for Phage Barco
Discovery Information
Isolation HostGordonia terrae 3612
Found ByKayla Albert
Year Found2016
Location FoundPittsburgh, PA USA
Finding InstitutionUniversity of Pittsburgh
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates40.447778 N, 79.959722 W Map
Discovery NotesThe phage was discovered in a garden outside of a Law Building. The soil sample the phage came from was collected during cool weather conditions (51 degrees Fahrenheit) and sunny. The weather conditions in the previous couple of days have been colder (high of 48 and low of 23 degrees Fahrenheit) and rainy.
Naming NotesThe name of this phage was inspired by the building it was found outside of, the Barco Law Building.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedMay 3, 2016
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage2927
Genome length (bp)48905
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceTCGCCGGTGA
GC Content67.3%
Fasta file available?Yes: Download fasta file
Characterization
ClusterCV
Subcluster--
Cluster Life CycleTemperate
Other Cluster Members
Annotating InstitutionGettysburg College
Annotation StatusIn GenBank
Plaque NotesThe plaque size ranges from 0.5mm to 2mm with the majority being on the larger size. The plaques have a halo shape with clear centers and cloudy outer rings (turbid)
MorphotypeSiphoviridae
Number of Genes77
Number of tRNAs0
Number of tmRNAs0
Has been Phamerated?Yes
Gene List
Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMK501730
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 1.46x10^11 pfu/mL
Date of SEA Lysate Titering Feb 22, 2016
Pitt Freezer Box# 3
Pitt Freezer Box Grid# C1
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
Final DNAMaster FileDownload
GenBank File for PhameratorDownload