Gordonia phage Blino
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Detailed Information for Phage Blino
Discovery Information
Isolation HostGordonia terrae 3612
Found ByAbigail Dean
Year Found2019
Location FoundPittsburgh, PA United States
Finding InstitutionUniversity of Pittsburgh
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation Temperature30°C
GPS Coordinates40.4464 N, 79.9553 W Map
Discovery NotesLocation - Oakland, Pennsylvania (40.4464, -79.9553)

Date - 2019.9.17

Time - (3:30 PM)

Weather Conditions - Partly Cloudy, Humidity 75%, Pressure 30.01 inHg, Recently rained, Air Quality Good, UV Index 5

Temperature - 78 degrees F

Surrounding Area - Soil under the mulch behind the Wyndham Hotel. It was a more moist patch of soil and found at the base of some landscaped bushes and grass.

Transport Conditions

Duration - 0.5 hours

Temperature - 70 degrees inside a backpack

Exposure to Sunlight - none
Naming NotesBlino means tired or irritating in Welsh, which is the experience I had in finding and purifying this phage.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedAug 20, 2020
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage1211
Genome length (bp)54168
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceTGGCCGGTGA
GC Content66.9%
Fasta file available?Yes: Download fasta file
Characterization
ClusterCV
Subcluster--
Cluster Life CycleTemperate
Other Cluster Members
Annotating InstitutionUniversity of Pittsburgh
Annotation StatusIn GenBank
Plaque NotesFully grown plaques are around 1.8mm in diameter. They are clear plaques, not cloudy or having a halo, with relatively definite circular edges. The shape is not entirely perfectly circular.
Number of Genes81
Number of tRNAs0
Number of tmRNAs0
Has been Phamerated?Yes
Gene List

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Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMW291016
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 1.6x10^9
Pitt Freezer Box# 113
Pitt Freezer Box Grid# G9
Available Files
Plaque PictureDownload
GenBank File for PhameratorDownload