| Detailed Information for Phage Brandonk123 |
| Discovery Information |
| Isolation Host | Gordonia terrae 3612 |
| Found By | Brandon Kretlow, Brooke Butkowski |
| Year Found | 2016 |
| Location Found | River Falls, WI USA |
| Finding Institution | University of Wisconsin-River Falls |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 29°C |
| GPS Coordinates | 44.8502 N, 92.624713 W Map |
| Discovery Notes | soil sample collected at 2:13pm under a bridge by the Uwrf sports center with moist and sandy soil conditions. at 10mm depth in 73°F in sunny conditions 54% humidity. This phage was discovered by Brooke and then further tested by Brandon in the lab |
| Naming Notes | Brandonk123 is Brandon's name + his birthday since he is bad at naming. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 23, 2017 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Approximate Shotgun Coverage | 1628 |
| Genome length (bp) | 58842 |
| Character of genome ends | Circularly Permuted |
| GC Content | 67.3% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | DE |
| Subcluster | DE1 |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | the plaques are in the shape of a semihalo and are about 4-5 mm in diameter |
| Morphotype | Siphoviridae |
| Number of Genes | 89 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MG812487 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
2.1x10^9 |
| Date of SEA Lysate Titering |
Oct 10, 2016 |
| Pitt Freezer Box# |
37 |
| Pitt Freezer Box Grid# |
E3 |
| Available Files |
| EM Picture | Download |
| GenBank File for Phamerator | Download |