Gordonia phage Charming
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Detailed Information for Phage Charming
Discovery Information
Isolation HostGordonia terrae 3612
Found ByNicholas Johnbosco
Year Found2018
Location FoundPittsburgh, PA USA
Finding InstitutionUniversity of Pittsburgh
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation Temperature30°C
GPS Coordinates40.44803 N, 79.956289 W Map
Discovery NotesThe soil was collected from the Litchfield towers in Pittsburgh PA on August 20, 2018. The weather outside was cloudy and cool with the temperature being about 82 degrees at the time of collection, 3:37 PM. The soil sample was transported for 5 mins and placed in the cool dark drawer in my desk. The soil was cold and slightly damp. There were small trees and shrubs near the collection site. The area was quite shady due to the towers shadow.
Naming NotesFrom a childhood fairy tale that I loved to read
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 15, 2019
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage1061
Genome length (bp)58000
Character of genome endsCircularly Permuted
GC Content67.1%
Fasta file available?Yes: Download fasta file
Characterization
ClusterDE
SubclusterDE1
Cluster Life CycleLytic
Lysogeny NotesPhage Charming used a standard protocol to test for lysogeny which resulted in clearings all over the plate, which indicates a phage with a lytic cycle
Other Cluster Members
Annotating InstitutionUniversity of Pittsburgh
Annotation StatusIn GenBank
Plaque NotesPhage Charming produces circular plaques that are about 1-5mm in diameter with the majority of them being around the 3mm.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

Click to View

Charming_1
(helix-turn-helix DNA binding domain protein)

Charming_2
(terminase)

Charming_3

Charming_4
(membrane protein)

Charming_5

Charming_6

Charming_7

Charming_8
(lipoprotein)

Charming_9
(membrane protein)

Charming_10
(portal protein)

Charming_11

Charming_12
(MuF-like minor capsid protein)

Charming_13

Charming_14
(capsid maturation protease)

Charming_15

Charming_16
(HNH endonuclease)

Charming_17

Charming_18
(major capsid protein)

Charming_19

Charming_20

Charming_21
(major tail protein)

Charming_22

Charming_23

Charming_24
(esterase)

Charming_25

Charming_26

Charming_27
(head-to-tail adaptor)

Charming_28

Charming_29

Charming_30

Charming_31
(tail assembly chaperone)

Charming_32
(tail assembly chaperone)

Charming_33
(tape measure protein)

Charming_34
(minor tail protein)

Charming_35
(minor tail protein)

Charming_36
(HNH endonuclease)

Charming_37

Charming_38
(minor tail protein)

Charming_39

Charming_40

Charming_41
(HNH endonuclease)

Charming_42
(lysin A)

Charming_43
(holin)

Charming_44
(membrane protein)

Charming_45
(membrane protein)

Charming_46
(lysin B)

Charming_47

Charming_48

Charming_49
(RecE-like exonuclease)

Charming_50

Charming_51

Charming_52
(RecT-like ssDNA binding protein)

Charming_53

Charming_54

Charming_55
(helix-turn-helix DNA binding domain protein)

Charming_56

Charming_57
(helix-turn-helix DNA binding domain protein)

Charming_58

Charming_59

Charming_60

Charming_61
(membrane protein)

Charming_62
(membrane protein)

Charming_63

Charming_64

Charming_65

Charming_66

Charming_67

Charming_68

Charming_69

Charming_70

Charming_71

Charming_72
(RuvC-like resolvase)

Charming_73

Charming_74

Charming_75

Charming_76

Charming_77

Charming_78

Charming_79

Charming_80
(DNA binding domain protein)

Charming_81

Charming_82

Charming_83

Charming_84

Charming_85

Charming_86

Charming_87
(helix-turn-helix DNA binding domain protein)

Charming_88
(methyltransferase)

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMK937601
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 3 * 10^10 pfu/mL
Pitt Freezer Box# 89
Pitt Freezer Box Grid# H11
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload