Rhodococcus phage Braxoaddie
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Detailed Information for Phage Braxoaddie
Discovery Information
Isolation HostRhodococcus equi NRRL B-16538
Found BySophia Doorn
Year Found2022
Location FoundGrand Rapids, MI United States
Finding InstitutionCalvin University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature28°C
GPS Coordinates42.932919 N, 85.597268 W Map
Discovery NotesThe sample was taken from about two inches into the ground. It was slight damp and sandy and found in an area that was shaded by brush.
Naming NotesI nanny two kids during the summer, Braxon and Haddie, and I combined their names to name the phage.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedDec 15, 2022
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina
Approximate Shotgun Coverage1933
Genome length (bp)67415
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceAGCCGCGTAC
GC Content67.3%
Fasta file available?Yes: Download fasta file
Characterization
ClusterCR
Subcluster--
Cluster Life CycleLytic
Other Cluster Members
Annotating InstitutionCalvin University
Annotation StatusIn GenBank
Plaque NotesBraxoaddie is a lytic phage because the plaques are clear, round, and have defined edges. The plaques were just above 1 mm in diameter.
MorphotypeSiphoviridae
Number of Genes96
Number of tRNAs0
Number of tmRNAs0
Has been Phamerated?Yes
Gene List

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Braxoaddie_1
(terminase small subunit)

Braxoaddie_2

Braxoaddie_3

Braxoaddie_4

Braxoaddie_5

Braxoaddie_6

Braxoaddie_7

Braxoaddie_8

Braxoaddie_9

Braxoaddie_10

Braxoaddie_11

Braxoaddie_12

Braxoaddie_13

Braxoaddie_14

Braxoaddie_15

Braxoaddie_16

Braxoaddie_17

Braxoaddie_18
(ThyX-like thymidylate synthase)

Braxoaddie_19

Braxoaddie_20
(lysin B)

Braxoaddie_21
(MazG-like nucleotide pyrophosphohydrolase)

Braxoaddie_22

Braxoaddie_23
(minor tail protein)

Braxoaddie_24

Braxoaddie_25

Braxoaddie_26

Braxoaddie_27
(HNH endonuclease)

Braxoaddie_28
(terminase large subunit)

Braxoaddie_29
(portal protein)

Braxoaddie_30

Braxoaddie_31
(scaffolding protein)

Braxoaddie_32
(capsid decoration protein)

Braxoaddie_33
(major capsid protein)

Braxoaddie_34

Braxoaddie_35
(head-to-tail adaptor)

Braxoaddie_36
(head-to-tail stopper)

Braxoaddie_37

Braxoaddie_38
(tail terminator)

Braxoaddie_39
(major tail protein)

Braxoaddie_40

Braxoaddie_41
(tail assembly chaperone)

Braxoaddie_42
(tail assembly chaperone)

Braxoaddie_43
(tape measure protein)

Braxoaddie_44
(minor tail protein)

Braxoaddie_45
(minor tail protein)

Braxoaddie_46
(minor tail protein)

Braxoaddie_47

Braxoaddie_48
(minor tail protein)

Braxoaddie_49

Braxoaddie_50
(lysin A)

Braxoaddie_51
(holin)

Braxoaddie_52

Braxoaddie_53
(DNA primase/polymerase)

Braxoaddie_54

Braxoaddie_55
(WhiB family transcription factor)

Braxoaddie_56

Braxoaddie_57
(helix-turn-helix DNA binding protein)

Braxoaddie_58
(RuvC-like resolvase)

Braxoaddie_59
(DnaE-like DNA polymerase III alpha)

Braxoaddie_60

Braxoaddie_61

Braxoaddie_62

Braxoaddie_63

Braxoaddie_64
(RecA-like DNA recombinase)

Braxoaddie_65

Braxoaddie_66

Braxoaddie_67
(Cas4 exonuclease)

Braxoaddie_68

Braxoaddie_69

Braxoaddie_70

Braxoaddie_71

Braxoaddie_72
(DNA helicase)

Braxoaddie_73

Braxoaddie_74

Braxoaddie_75
(RtcB-like RNA ligase)

Braxoaddie_76

Braxoaddie_77

Braxoaddie_78
(DNA binding protein)

Braxoaddie_79

Braxoaddie_80

Braxoaddie_81

Braxoaddie_82

Braxoaddie_83

Braxoaddie_84

Braxoaddie_85

Braxoaddie_86

Braxoaddie_87

Braxoaddie_88

Braxoaddie_89

Braxoaddie_90

Braxoaddie_91

Braxoaddie_92

Braxoaddie_93

Braxoaddie_94

Braxoaddie_95

Braxoaddie_96

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionOQ938585
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 162
Pitt Freezer Box Grid# E2
Available Files
Plaque PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload