| Detailed Information for Phage Braxoaddie |
| Discovery Information |
| Isolation Host | Rhodococcus equi NRRL B-16538 |
| Found By | Sophia Doorn |
| Year Found | 2022 |
| Location Found | Grand Rapids, MI United States |
| Finding Institution | Calvin University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 28°C |
| GPS Coordinates | 42.932919 N, 85.597268 W Map |
| Discovery Notes | The sample was taken from about two inches into the ground. It was slight damp and sandy and found in an area that was shaded by brush. |
| Naming Notes | I nanny two kids during the summer, Braxon and Haddie, and I combined their names to name the phage. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Dec 15, 2022 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 1933 |
| Genome length (bp) | 67415 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | AGCCGCGTAC |
| GC Content | 67.3% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | CR |
| Subcluster | CR6 |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | Calvin University |
| Annotation Status | In GenBank |
| Plaque Notes | Braxoaddie is a lytic phage because the plaques are clear, round, and have defined edges. The plaques were just above 1 mm in diameter. |
| Morphotype | Siphoviridae |
| Number of Genes | 96 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | OQ938585 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
162 |
| Pitt Freezer Box Grid# |
E2 |
| Available Files |
| Plaque Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
