| Detailed Information for Phage CaptainKirk2 |
| Discovery Information |
| Isolation Host | Gordonia terrae 3612 |
| Found By | Charu Arora |
| Year Found | 2015 |
| Location Found | Pittsburgh, PA USA |
| Finding Institution | University of Pittsburgh |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 30°C |
| GPS Coordinates | 40.44574 N, 79.95372 W Map |
| Discovery Notes | The soil sample was collected near a flower garden, it was cloudy and 70 degrees. I adopted this from Alison Hoke's soil sample and original plate. |
| Naming Notes | I chose to name this star trek, as Ali and I had decided together to name hers Captain Janeway following the idea of mystery presented by the phages and the urge I have to explore and understand my phage, similar to Captain Kirk. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 27, 2016 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 3241 |
| Genome length (bp) | 47898 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | TCGCCGGTGA |
| GC Content | 67.4% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | CV |
| Subcluster | -- |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | There were two phages from the sample Ali collected. She collected the smaller plaque and named it (Captain Janeway) and I collected the larger plaque, which became my phage. Originally I thought I had 2 morphologies on my plaque, but I didn't after multiple tests. |
| Morphotype | Siphoviridae |
| Number of Genes | 79 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | KX557274 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
14.7*10^-9 |
| Date of SEA Lysate Titering |
Nov 19, 2015 |
| Pitt Freezer Box# |
2 |
| Pitt Freezer Box Grid# |
D6 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| Final DNAMaster File | Download |
