| Detailed Information for Phage Duocatuli |
| Discovery Information |
| Isolation Host | Microbacterium foliorum NRRL B-24224 |
| Found By | Natalie Schneider |
| Year Found | 2021 |
| Location Found | Washington, MO United States |
| Finding Institution | St. Louis Community College |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 38.546624 N, 91.01274 W Map |
| Discovery Notes | The sample spot receives morning shade, and afternoon sun. The sample was mostly dry and crumbly. Sample was collected from a six inch depth and stored in a ziplock bag.
Sample was taken at 7:30 am on a cool winters morning (about 40 degrees F) and stored in a refrigerator until I took my hour commute to school at 12:00 pm. |
| Naming Notes | The phage name is latin for "Two puppies". I discovered Duocatuli from a sample taken from a hole that my two puppies dug in my yard. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jun 24, 2021 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 177 |
| Genome length (bp) | 41551 |
| Character of genome ends | Circularly Permuted |
| GC Content | 63.4% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | EA |
| Subcluster | EA1 |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | St. Louis Community College |
| Annotation Status | In GenBank |
| Plaque Notes | During the purification process plaques varied in size and number. The plaques were always temperate turbid and often displayed a surrounding halo. |
| Morphotype | Siphoviridae |
| Number of Genes | 62 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | ON260823 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
158 |
| Pitt Freezer Box Grid# |
A2 |
| Available Files |
| Plaque Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
