| Detailed Information for Phage Gambino |
| Discovery Information |
| Isolation Host | Gordonia terrae 3612 |
| Duplicates of this Phage | MattSquared |
| Found By | Neha Gupta |
| Year Found | 2017 |
| Location Found | Pittsburgh, PA USA |
| Finding Institution | University of Pittsburgh |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Unknown |
| Isolation Temperature | Not entered |
| GPS Coordinates | 40.44 N, 79.96 W Map |
| Discovery Notes | I discovered this phage from a soil sample collected on Sunday, January 8, 2017 outside Mark A. Nordenberg Hall. The weather was cold around 35 degrees fahrenheit, and the top of the soil was covered in snow with certain areas frozen. |
| Naming Notes | I wanted him to have a fun name since he's an enjoyable phage I think. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | May 22, 2017 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 1511 |
| Genome length (bp) | 53622 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | TGGCCGGTGA |
| GC Content | 67.1% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | CV |
| Subcluster | -- |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | University of Pittsburgh |
| Annotation Status | In GenBank |
| Plaque Notes | The plaques from this phage make relatively small clearings around 2mm in size. They are all roughly the same circular shape. Also, they are clear rather than cloudy in appearance. |
| Morphotype | Siphoviridae |
| Number of Genes | 88 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MN586031 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
55 |
| Pitt Freezer Box Grid# |
F12 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| Final DNAMaster File | Download |
| GenBank File for Phamerator | Download |
