The Actinobacteriophage Database

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Detailed Information for Phage HerculesXL
Discovery Information
Isolation Host	Microbacterium foliorum NRRL B-24224
Found By	Rebecca Meisner
Year Found	2021
Location Found	Corvallis, OR USA
Finding Institution	Oregon Coast Community College
Program	Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?	Yes
Isolation Temperature	30°C
GPS Coordinates	44.55003 N, 123.26578 W Map
Discovery Notes	The sample was collected from under a shrub outside of an animal hospital were dog could possibly be using the bathroom. The temperature was in the 60's and the ground was wet.
Naming Notes	I named my phage HerculesXL because I have hopes that in the future he will be used to fight antibiotic resistant bacteria and help save lives. I added XL on the end of the name because Hercules was already taken.
Sequencing Information
Sequencing Complete?	Yes
Date Sequencing Completed	Jan 17, 2022
Sequencing Facility	Pittsburgh Bacteriophage Institute
Shotgun Sequencing Method	Illumina
Sequencer Used	Illumina MiSeq
Read Type	Single-end reads
Read Length	150 bp
Approximate Shotgun Coverage	1853
Genome length (bp)	39410
Character of genome ends	Circularly Permuted
GC Content	63.9%
Fasta file available?	Yes: Download fasta file
Characterization
Cluster	EA
Subcluster	EA11
Cluster Life Cycle	Lytic
Other Cluster Members	Click to View Mercedes (None) Acosta (EA1) Agente005 (EA1) AlexAdler (EA1) Alyxandracam (EA1) Anilorac (EA1) Antoinette (EA1) AranulaLuti (EA1) Asta (EA1) Aubergine (EA1) AxiPup (EA1) Baines (EA1) Balsa (EA1) Bandik (EA1) BeautPeep30 (EA1) BeeBee8 (EA1) Benjalauren (EA1) BigRedClifford (EA1) Blage (EA1) BonesMcCoy (EA1) Bonino (EA1) Byron23 (EA1) Calix (EA1) CarisSwetlik (EA1) Chako (EA1) Chamuel (EA1) Christoph (EA1) Clancy (EA1) Convict (EA1) Dave (EA1) Delphidian (EA1) Den3 (EA1) Dothraki (EA1) Duocatuli (EA1) Endor (EA1) Erla (EA1) Espinosa (EA1) Etna (EA1) Etta (EA1) Exploradora (EA1) FarmerDoug (EA1) Figueroism (EA1) GardenB (EA1) Garey24 (EA1) Gargoyle (EA1) Gelo (EA1) Gershwin (EA1) Greys (EA1) Gubbabump (EA1) Hamlet (EA1) HankSprout (EA1) HanSolo (EA1) HungryHenry (EA1) Ilzat (EA1) Inventa (EA1) Ioannes (EA1) Janus167 (EA1) JasonD (EA1) JasperRussell (EA1) Jenos (EA1) Jerbirus (EA1) JeriBeth (EA1) Kale (EA1) KannH (EA1) KingJulien (EA1) Klimt (EA1) Knox (EA1) Kurt1 (EA1) Leafus (EA1) Lovelyunicorn (EA1) Ludgate (EA1) MaeLinda (EA1) ManRay (EA1) Martin (EA1) McGalleon (EA1) MillyPhilly (EA1) MonChoix (EA1) MrWorldwide (EA1) Nagem (EA1) Nattles (EA1) NickSell (EA1) Oats (EA1) OldNelly (EA1) Oxtober96 (EA1) Papafritta (EA1) ParleG (EA1) Peep (EA1) Peppino (EA1) Pherferi (EA1) Phiderman (EA1) Phireproof (EA1) PhredFlintston (EA1) Phriends (EA1) Pocket (EA1) PuppyEggo (EA1) Raccoon (EA1) Rapheph (EA1) Raptor (EA1) Redfield (EA1) Renzie (EA1) Riyhil (EA1) Robinson (EA1) Rog141 (EA1) RosieMae (EA1) Schnapsidee (EA1) Sedgewig (EA1) ShaiHulud (EA1) Shee (EA1) SJay (EA1) SoilGremlin (EA1) SonOfLevi (EA1) Stanktossa (EA1) StingRay (EA1) StirfryIV (EA1) Stormbreaker8 (EA1) Strathdee (EA1) Superfresh (EA1) Symere (EA1) TatarkaPM (EA1) Teagan (EA1) TeddyBear (EA1) Tenda (EA1) Thompsone (EA1) Thorongil (EA1) TinSulphur (EA1) Velene (EA1) Vispistious (EA1) WildNOut (EA1) Winzigespinne (EA1) Zada (EA1) Carostasia (EA10) HairyMan (EA10) Juanyo (EA10) Mandalorian (EA10) Nucci (EA10) Quartz (EA10) YuuY (EA10) HerculesXL (EA11) Ixel (EA11) Kavo (EA11) Schimmels22 (EA11) Scissor2024 (EA11) Tinyman4 (EA11) WestPM (EA11) Andromedas (EA2) ChikPic (EA2) ColaCorta (EA2) Eleri (EA2) Finny (EA2) Glamour (EA2) MCubed (EA2) Phanita (EA2) PSirce (EA2) Sansa (EA2) Saratos (EA2) Shamu (EA2) Xebius (EA2) Zenitsu (EA2) Casey (EA3) Pajaza (EA3) Pikmin (EA3) BouleyBill (EA4) Bustleton (EA4) Golden (EA4) Guetzie (EA4) Jingles (EA4) Kauala (EA4) Koji (EA4) Lucky3 (EA4) Pherbot (EA4) PrincePhergus (EA4) RenegadeRaider (EA4) Sinatra (EA4) SirVictor (EA4) Alakazam (EA5) BaronJohn (EA5) CaptainRex (EA5) Clock (EA5) Fulton (EA5) GreenIvy (EA5) Hasitha (EA5) Librie (EA5) LilTerminator (EA5) Nebulous (EA5) Neferthena (EA5) QuadZero (EA5) TwoBits (EA5) Wardwill (EA5) Zayuliv (EA5) Zepp (EA5) Chepli (EA6) Jemerald (EA6) Juicer (EA6) KatChan (EA6) Luna18 (EA6) Morrigan (EA6) WilliamStrong (EA6) Theresita (EA7) Schubert (EA8) Cheeto1 (EA9) ChickenKing (EA9) GaeCeo (EA9)
Annotating Institution	Oregon Coast Community College
Annotation Status	In GenBank
Number of Genes	60
Number of tRNAs	0
Number of tmRNAs	0
Has been Phamerated?	Yes
Gene List	Click to View HerculesXL_1 HerculesXL_2 (terminase) HerculesXL_3 HerculesXL_4 (portal protein) HerculesXL_5 HerculesXL_6 HerculesXL_7 (minor capsid protein) HerculesXL_8 (scaffolding protein) HerculesXL_9 (major capsid protein) HerculesXL_10 HerculesXL_11 HerculesXL_12 (minor capsid protein) HerculesXL_13 (tail terminator) HerculesXL_14 HerculesXL_15 (major tail protein) HerculesXL_16 (tail assembly chaperone) HerculesXL_17 (tail assembly chaperone) HerculesXL_18 (tape measure protein) HerculesXL_19 (minor tail protein) HerculesXL_20 (minor tail protein) HerculesXL_21 HerculesXL_22 HerculesXL_23 (endolysin) HerculesXL_24 HerculesXL_25 HerculesXL_26 HerculesXL_27 HerculesXL_28 HerculesXL_29 HerculesXL_30 HerculesXL_31 HerculesXL_32 (DNA primase/helicase) HerculesXL_33 (VRR-Nuc domain protein) HerculesXL_34 HerculesXL_35 (AAA-ATPase) HerculesXL_36 (RecB-like exonuclease/helicase) HerculesXL_37 (DNA polymerase I) HerculesXL_39 HerculesXL_40 (DNA helicase) HerculesXL_41 (MazG-like nucleotide pyrophosphohydrolase) HerculesXL_42 HerculesXL_43 (thymidylate kinase) HerculesXL_44 (glycosyltransferase) HerculesXL_45 HerculesXL_46 HerculesXL_47 (thymidylate synthase) HerculesXL_48 HerculesXL_49 HerculesXL_50 HerculesXL_51 HerculesXL_52 HerculesXL_53 HerculesXL_54 HerculesXL_55 HerculesXL_56 HerculesXL_57 HerculesXL_58 HerculesXL_59 HerculesXL_60 HerculesXL_61
Submitted Annotation in DNA Master Format	Download
Submitted Minimal DNA Master File	Download
Author List	Download
Annotation Cover Sheet	Download
Publication Info
Uploaded to GenBank?	Yes
GenBank Accession	OP068330
Refseq Number	None yet
Published in Paper	Fisher et al 2022, Microbiology Resource Announcements: Complete Annotated Genome Sequences of Two Novel Microbacteriophages, Gingerbug and HerculesXL, from Western Oregon, USA
Archiving Info
Archiving status	Archived
Pitt Freezer Box#	143
Pitt Freezer Box Grid#	B1
Available Files
EM Picture	Download
GenBank File for Phamerator	Download

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