| Detailed Information for Phage Kabluna |
| Discovery Information |
| Isolation Host | Gordonia terrae 3612 |
| Found By | Solomon Klombers |
| Year Found | 2016 |
| Location Found | Pittsburgh, PA USA |
| Finding Institution | University of Pittsburgh |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 30°C |
| GPS Coordinates | 40.441111 N, 79.954167 W Map |
| Discovery Notes | Soil sample was retrieved from a Garden box outside of Wesley W. Posvar Hall. Temperature at time of collection was 61 degrees Fahrenheit. Weather conditions in the previous 24 hours were in the mid 40s and clear. Soil sample was collected on January 31st, 2016. Plaques were found using a direct plating procedure. |
| Naming Notes | The name "Kabluna" is an Ihalmiut word for 'invader,' or 'blight.' The Ihalmiut, literally "People from beyond," were a tribe of Inuit Indians who lived in Canada's Kivalliq region, present day Nunavut. The Ihalmiut used the word "Kabluna" to describe the diseases (and those who brought disease to them) which ultimately ended their way of life. As such, I thought the name 'Kabluna' apt for a bacteriophage, seeing as they are, from the perspective of the bacterial hosts, also 'invaders,' and 'blights.' |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jul 6, 2016 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 3235 |
| Genome length (bp) | 65869 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGCCGCGTAC |
| GC Content | 65.6% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | CR |
| Subcluster | CR2 |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | It appears that Kabluna may have two morphologies. There are lager plaques, roughly 1/10th of a cm in diameter, and smaller plaques roughly 1mm in diameter. |
| Morphotype | Siphoviridae |
| Number of Genes | 95 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MF919510 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
3 |
| Pitt Freezer Box Grid# |
D10 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
