| Discovery Information |
| Isolation Host | Gordonia rubripertincta NRRL B-16540 |
| Duplicates of this Phage | Montero, UziFAB |
| Found By | Sophie Grace, Palmer Wright, Catherine Loftus |
| Year Found | 2021 |
| Location Found | Lincolm, NE United States |
| Finding Institution | University of Nebraska-Lincoln |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 40.81937 N, 96.69705 W Map |
| Discovery Notes | These phage were found in the backyard of the Kappa Kappa Gamma house on UNL's campus. It was found in the middle of a construction site. It was 2.5 inches deep, and the ambient temperature was 85 degrees (Farenheit). |
| Naming Notes | We are all in a sorority/fraternity, so we combined Kappa Kappa Gamma, Farmhouse, and Kappa Delta. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 21, 2022 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 1075 |
| Genome length (bp) | 49606 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | TCGCCGGTGA |
| GC Content | 67.3% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | CV |
| Subcluster | -- |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | University of Nebraska-Lincoln |
| Annotation Status | In GenBank |
| Plaque Notes | We believe we have a temperate phage, since the plaques are turbid. |
| Number of Genes | 81 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | ON970567 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
137 |
| Pitt Freezer Box Grid# |
F7 |
| Available Files |
| GenBank File for Phamerator | Download |