| Detailed Information for Phage Lennon |
| Discovery Information |
| Isolation Host | Gordonia terrae 3612 |
| Found By | Jake Breimann |
| Year Found | 2015 |
| Location Found | Pittsburgh, PA USA |
| Finding Institution | University of Pittsburgh |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 37°C |
| GPS Coordinates | 40.444800 N, 79.952700 W Map |
| Discovery Notes | Weather Conditions at time of collection: 45 degrees F, cloudy
Soil Conditions: slightly muddy, hard
Soil collected directly across from Clapp Hall near sidewalk leading to Cathedral of Learning. |
| Naming Notes | Since the phage had to be adopted from another student, I named the phage Lennon as in John Lennon because he was also adopted. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Apr 26, 2016 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Approximate Shotgun Coverage | 1684 |
| Genome length (bp) | 60237 |
| Character of genome ends | Circularly Permuted |
| GC Content | 67.4% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | DE |
| Subcluster | DE1 |
| Cluster Life Cycle | Lytic |
| Lysogeny Notes | N/A |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | The plaques are oval-like clearings and relatively small (2 mm). From the observed plaques, only one phage was present on the plate due to plaque consistency. The phage is highly lytic due to clear plaques being suspended on the plate. |
| Morphotype | Siphoviridae |
| Number of Genes | 85 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MF919514 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Ignore |
| SEA Lysate Titer |
3*10^(12) |
| Date of SEA Lysate Titering |
Mar 30, 2016 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| Final DNAMaster File | Download |
| GenBank File for Phamerator | Download |
