Gordonia phage Lennon
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Detailed Information for Phage Lennon
Discovery Information
Isolation HostGordonia terrae 3612
Found ByJake Breimann
Year Found2015
Location FoundPittsburgh, PA USA
Finding InstitutionUniversity of Pittsburgh
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature37°C
GPS Coordinates40.444800 N, 79.952700 W Map
Discovery NotesWeather Conditions at time of collection: 45 degrees F, cloudy
Soil Conditions: slightly muddy, hard
Soil collected directly across from Clapp Hall near sidewalk leading to Cathedral of Learning.
Naming NotesSince the phage had to be adopted from another student, I named the phage Lennon as in John Lennon because he was also adopted.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedApr 26, 2016
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage1684
Genome length (bp)60237
Character of genome endsCircularly Permuted
GC Content67.4%
Fasta file available?Yes: Download fasta file
Characterization
ClusterDE
SubclusterDE1
Cluster Life CycleLytic
Lysogeny NotesN/A
Other Cluster Members
Annotating InstitutionUnknown or unassigned
Annotation StatusIn GenBank
Plaque NotesThe plaques are oval-like clearings and relatively small (2 mm). From the observed plaques, only one phage was present on the plate due to plaque consistency. The phage is highly lytic due to clear plaques being suspended on the plate.
MorphotypeSiphoviridae
Number of Genes85
Number of tRNAs0
Number of tmRNAs0
Has been Phamerated?Yes
Gene List
Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMF919514
Refseq NumberNone yet
Archiving Info
Archiving status Ignore
SEA Lysate Titer 3*10^(12)
Date of SEA Lysate Titering Mar 30, 2016
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
Final DNAMaster FileDownload
GenBank File for PhameratorDownload