| Detailed Information for Phage MerCougar |
| Discovery Information |
| Isolation Host | Gordonia terrae 3612 |
| Former names | SBNMerCougar |
| Found By | Brendan Moline |
| Year Found | 2019 |
| Location Found | Bangor, ME United States |
| Finding Institution | University of Maine, Honors College |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 16°C |
| GPS Coordinates | 44.805653 N, 68.763775 W Map |
| Discovery Notes | This phage was found in the middle of a grass lawn, so there was a lot of organic material in the sample. The soil was not too moist and the air was of a moderate humidity. The soil depth was 0" to 0.50" deep. Collected in the early evening hours. |
| Naming Notes | This phage was discovered by my Catholic elementary school, with SBN representing Saint Brendan the Navigator. The MerCougar part comes from the car that I drive, which is a late-1970s Mercury Cougar. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 5, 2021 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Approximate Shotgun Coverage | 204 |
| Genome length (bp) | 68839 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGCCGCGTAC |
| GC Content | 65.5% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | CR |
| Subcluster | CR2 |
| Cluster Life Cycle | Lytic |
| Lysogeny Notes | Does not perform lysogeny |
| Other Cluster Members |
|
| Annotating Institution | Saint Joseph's University |
| Annotation Status | In GenBank |
| Plaque Notes | The plaques are about 3-4 millimeters in diameter, with a turbid halo-like ring around a cleared center point. |
| Morphotype | Siphoviridae |
| Number of Genes | 94 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | OQ709223 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
109 |
| Pitt Freezer Box Grid# |
F11 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| GenBank File for Phamerator | Download |
