| Detailed Information for Phage Nattles |
| Discovery Information |
| Isolation Host | Microbacterium foliorum NRRL B-24224 |
| Found By | Audrey Jonas |
| Year Found | 2016 |
| Location Found | Pittsburgh, PA USA |
| Finding Institution | University of Pittsburgh |
| Program | Phage Hunters Integrating Research and Education |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 40.444859 N, 79.952833 W Map |
| Discovery Notes | Soil was found from a pile of construction dirt around the Cathedral of Learning a day after it rained. Temperature was around 26 degrees Celsius. |
| Naming Notes | This is my younger sister's nickname |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Sep 19, 2017 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 2245 |
| Genome length (bp) | 41544 |
| Character of genome ends | Circularly Permuted |
| GC Content | 63.4% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | EA |
| Subcluster | EA1 |
| Cluster Life Cycle | Lytic |
| Lysogeny Notes | Growth of mesas and lysogeny efficiency using phage-seeded plates was attempted with this phage by Audrey Jonas. This phage does not make a lysogen. |
| Other Cluster Members |
|
| Annotating Institution | University of Pittsburgh |
| Annotation Status | In GenBank |
| Plaque Notes | Plaques are haloed and clear and about 1 mm in diameter, appearing after 18-24 hours of incubation at 30 degrees Celsius followed by 6 hours of incubation at room temp, approx. 25 degrees Celsius |
| Morphotype | Siphoviridae |
| Number of Genes | 62 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MG925352 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
5 |
| Pitt Freezer Box Grid# |
C2 |
| Available Files |
| EM Picture | Download |
| Final DNAMaster File | Download |
| GenBank File for Phamerator | Download |