| Detailed Information for Phage Obliviate |
| Discovery Information |
| Isolation Host | Gordonia terrae 3612 |
| Found By | Taylor Morgan |
| Year Found | 2015 |
| Location Found | Pittsburgh, PA USA |
| Finding Institution | University of Pittsburgh |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 29°C |
| GPS Coordinates | 40.4419 N, 79.9557 W Map |
| Discovery Notes | The soil came from a flower bed with some trees nearby. It was a hot day around 84 degrees, dry. The soil was fairly dark. |
| Naming Notes | The Harry Potter series inspired me to name this phage. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Nov 13, 2015 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 619 |
| Genome length (bp) | 49286 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | TCTCCGGTGA |
| GC Content | 67.5% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | CV |
| Subcluster | -- |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | Obliviate has 2 different morphologies. One is large and haloed, about 3 mm and the other is small and clear, about 1 mm. |
| Number of Genes | 80 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | KU963254 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
7*10^10 |
| Date of SEA Lysate Titering |
Oct 13, 2015 |
| Pitt Freezer Box# |
2 |
| Pitt Freezer Box Grid# |
G12 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| Final DNAMaster File | Download |