Gordonia phage Obliviate
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Detailed Information for Phage Obliviate
Discovery Information
Isolation HostGordonia terrae 3612
Found ByTaylor Morgan
Year Found2015
Location FoundPittsburgh, PA USA
Finding InstitutionUniversity of Pittsburgh
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature29°C
GPS Coordinates40.4419 N, 79.9557 W Map
Discovery NotesThe soil came from a flower bed with some trees nearby. It was a hot day around 84 degrees, dry. The soil was fairly dark.
Naming NotesThe Harry Potter series inspired me to name this phage.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedNov 13, 2015
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage619
Genome length (bp)49286
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceTCTCCGGTGA
GC Content67.5%
Fasta file available?Yes: Download fasta file
Characterization
ClusterCV
Subcluster--
Cluster Life CycleTemperate
Other Cluster Members
Annotating InstitutionUnknown or unassigned
Annotation StatusIn GenBank
Plaque NotesObliviate has 2 different morphologies. One is large and haloed, about 3 mm and the other is small and clear, about 1 mm.
Number of Genes80
Number of tRNAs0
Number of tmRNAs0
Has been Phamerated?Yes
Gene List

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Obliviate_1
(terminase small subunit)

Obliviate_2
(terminase large subunit)

Obliviate_3
(portal protein)

Obliviate_4
(capsid maturation protease)

Obliviate_5
(scaffolding protein)

Obliviate_6
(major capsid protein)

Obliviate_7

Obliviate_8
(head-to-tail adaptor)

Obliviate_9
(head-to-tail stopper)

Obliviate_10

Obliviate_11

Obliviate_12
(tail terminator)

Obliviate_13
(major tail protein)

Obliviate_14
(tail assembly chaperone)

Obliviate_15
(tail assembly chaperone)

Obliviate_16
(tape measure protein)

Obliviate_17
(minor tail protein)

Obliviate_18
(minor tail protein)

Obliviate_19
(lysin A, L-Ala-D-Glu peptidase domain)

Obliviate_20
(lysin A, glycosyl hydrolase domain)

Obliviate_21

Obliviate_22

Obliviate_23

Obliviate_24

Obliviate_25
(lysin B)

Obliviate_26

Obliviate_27
(minor tail protein)

Obliviate_28
(minor tail protein)

Obliviate_29

Obliviate_30
(minor tail protein)

Obliviate_31
(minor tail protein)

Obliviate_32

Obliviate_33

Obliviate_34

Obliviate_35

Obliviate_36
(tyrosine integrase)

Obliviate_37

Obliviate_38
(immunity repressor)

Obliviate_39
(helix-turn-helix DNA binding protein)

Obliviate_40

Obliviate_41

Obliviate_42

Obliviate_43

Obliviate_44

Obliviate_45

Obliviate_46

Obliviate_47

Obliviate_48

Obliviate_49

Obliviate_50

Obliviate_51

Obliviate_52

Obliviate_53

Obliviate_54
(exonuclease)

Obliviate_55
(RecT-like ssDNA binding protein)

Obliviate_56

Obliviate_57

Obliviate_58

Obliviate_59

Obliviate_60

Obliviate_61

Obliviate_62
(nucleotidyl transferase)

Obliviate_63

Obliviate_64

Obliviate_65

Obliviate_66

Obliviate_67

Obliviate_68

Obliviate_69

Obliviate_70

Obliviate_71

Obliviate_72

Obliviate_73

Obliviate_74
(RuvC-like resolvase)

Obliviate_75

Obliviate_76

Obliviate_77

Obliviate_78

Obliviate_79

Obliviate_80
(HNH endonuclease)

Publication Info
Uploaded to GenBank?Yes
GenBank AccessionKU963254
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 7*10^10
Date of SEA Lysate Titering Oct 13, 2015
Pitt Freezer Box# 2
Pitt Freezer Box Grid# G12
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
Final DNAMaster FileDownload