| Detailed Information for Phage Patio | |
| Discovery Information | |
| Isolation Host | Gordonia terrae 3612 |
| Former names | TeaPea |
| Found By | Sarah Kuchan |
| Year Found | 2016 |
| Location Found | Pittsburgh, PA USA |
| Finding Institution | University of Pittsburgh |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 40.442840, -79.956205 Map |
| Discovery Notes | -at time of soil collection it was a dry overcast day and was 26 F -The soil was frozen -the weather conditions over the last 24 hours were around 10 F and windy + overcast -the soil was collected less than an hour before class (this is new) -the soil had fertilizer pellets (blue dots) on it |
| Naming Notes | Originally, I wanted to name him TP because I found him on the patio of one of our residence halls- Towers Patio. However, when I read the naming rules I saw the names could not be only 2 letters, so I extended it to TeePee (I still get the same effect). Then, that name was already taken. To combat this catastrophe, I changed it to TeaPea. Like the tea you drink + peas you eat. Very clever. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jun 30, 2016 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Approximate Shotgun Coverage | 3811 |
| Genome length (bp) | 66251 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGCCGCGTAC |
| GC Content | 65.6% |
| Sequencing Notes | 22% of reads call 10 G's instead of consensus 9 between 65907 - 65917 bp |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | CR |
| Subcluster | CR3 |
| Cluster Life Cycle | Lytic |
| Lysogeny Notes | -temperate phage |
| Other Cluster Members |
Click to ViewApiary (None) Braxoaddie (None) CoffeeBean (None) GuyFagieri (None) MacGully (None) Maselop (None) Polyyuki (None) Dalilpop (CR1) Flapper (CR1) GRU1 (CR1) GTE5 (CR1) Turuncu (CR1) Arti (CR2) Bonum (CR2) Buggaboo (CR2) Commandaria (CR2) Emianna (CR2) Foxboro (CR2) GrootJr (CR2) GTE8 (CR2) Jifall16 (CR2) Kabluna (CR2) KidneyBean (CR2) Kurt (CR2) MerCougar (CR2) NatB6 (CR2) NosilaM (CR2) NovumRegina (CR2) Outis (CR2) Phomeo (CR2) StarStruck (CR2) SuperSulley (CR2) Tracker (CR2) Wheezy (CR2) Ennea (CR3) Float294 (CR3) Lollipop1437 (CR3) Patio (CR3) Skysand (CR3) BiPauneto (CR4) HubbaBubba (CR4) IDyn (CR4) Marietta (CR4) NadineRae (CR4) Sukkupi (CR4) WhoseManz (CR4) Yndexa (CR4) Fury (CR5) Pleakley (CR5) |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | -large plaques -halos in all of them -range from 1 mm- 3mm. |
| Morphotype | Siphoviridae |
| Number of Genes | 90 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewPatio_1 Patio_2 Patio_3 Patio_4 Patio_5 Patio_6 Patio_7 Patio_8 Patio_9 Patio_10 Patio_11 Patio_12 Patio_13 Patio_14 Patio_15 Patio_16 Patio_17 Patio_18 Patio_19 Patio_20 Patio_21 Patio_22 Patio_23 Patio_24 Patio_25 Patio_26 Patio_27 Patio_28 Patio_29 Patio_30 Patio_31 Patio_32 Patio_33 Patio_34 Patio_35 Patio_36 Patio_37 Patio_38 Patio_39 Patio_40 Patio_41 Patio_42 Patio_43 Patio_44 Patio_45 Patio_46 Patio_47 Patio_48 Patio_49 Patio_50 Patio_51 Patio_52 Patio_53 Patio_54 Patio_55 Patio_56 Patio_57 Patio_58 Patio_59 Patio_60 Patio_61 Patio_62 Patio_63 Patio_64 Patio_65 Patio_66 Patio_67 Patio_68 Patio_69 Patio_70 Patio_71 Patio_72 Patio_73 Patio_74 Patio_75 Patio_76 Patio_77 Patio_78 Patio_79 Patio_80 Patio_81 Patio_82 Patio_83 Patio_84 Patio_85 Patio_86 Patio_87 Patio_88 Patio_89 Patio_90 |
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MF919542 |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| SEA Lysate Titer | 8.0x10^9 |
| Date of SEA Lysate Titering | Feb 29, 2016 |
| Pitt Freezer Box# | 3 |
| Pitt Freezer Box Grid# | F7 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
