Detailed Information for Phage PhrostedPhlake |
Discovery Information |
Isolation Host | Gordonia terrae 3612 |
Found By | Deborah Lee |
Year Found | 2017 |
Location Found | Pittsburgh, PA USA |
Finding Institution | University of Pittsburgh |
Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
From enriched soil sample? | No |
Isolation Temperature | Not entered |
GPS Coordinates | 40.443558 N, 79.960855 W Map |
Discovery Notes | I collected this sample from a garden bed outside of Scaife Hall at the University of Pittsburgh. The soil was moist, soft, and easy to collect. The weather conditions at the time of collection were 12°C, overcast, and not windy at all. The weather conditions in the last 24 hours were light showers with a high of 4°C and a low of -3°C. Sample was transported for 30 minutes prior to storage and stored for 1 hour in a desk drawer at 70°C. |
Naming Notes | This phage is named after Kellogg's Frosted Flakes cereal. |
Sequencing Information |
Sequencing Complete? | Yes |
Date Sequencing Completed | May 17, 2017 |
Sequencing Facility | Pittsburgh Bacteriophage Institute |
Shotgun Sequencing Method | Illumina Sequencing |
Approximate Shotgun Coverage | 1954 |
Genome length (bp) | 52042 |
Character of genome ends | 3' Sticky Overhang |
Overhang Length | 10 bases |
Overhang Sequence | TCTCCGGTGA |
GC Content | 67.4% |
Fasta file available? | Yes: Download fasta file |
Characterization |
Cluster | CV |
Subcluster | -- |
Cluster Life Cycle | Temperate |
Other Cluster Members |
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Annotating Institution | University of Pittsburgh |
Annotation Status | In GenBank |
Plaque Notes | The plaques are all tiny, clear, and circular, and the morphology is very consistent. Almost all of the plaques have a diameter of 1mm. |
Number of Genes | 88 |
Number of tRNAs | 0 |
Number of tmRNAs | 0 |
Has been Phamerated? | Yes |
Gene List |
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Submitted Minimal DNA Master File | Download |
Publication Info |
Uploaded to GenBank? | Yes |
GenBank Accession | MK801724 |
Refseq Number | None yet |
Archiving Info |
Archiving status |
Archived |
SEA Lysate Titer |
5E10 |
Pitt Freezer Box# |
56 |
Pitt Freezer Box Grid# |
B7 |
Available Files |
Plaque Picture | Download |
Restriction Digest Picture | Download |
EM Picture | Download |
GenBank File for Phamerator | Download |