| Detailed Information for Phage Polyyuki |
| Discovery Information |
| Isolation Host | Rhodococcus equi NRRL B-16538 |
| Found By | Matt Minasian |
| Year Found | 2022 |
| Location Found | Grand Rapids, MI United States |
| Finding Institution | Calvin University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Unknown |
| Isolation Temperature | Not entered |
| GPS Coordinates | 42.933366 N, 85.58658 W Map |
| Discovery Notes | Sample was taken up a kept-up garden area with plants. Dirt was dry. Isolated it after 3 tries since the first 2 tries seemed to have different plaques. |
| Naming Notes | Named after 2 of my pets. I have a dog named Polly and a cat named Yuki, so the name is a combination of both of their names. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Mar 14, 2023 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 3660 |
| Genome length (bp) | 67447 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | AGCCGCGTAC |
| GC Content | 67.3% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | CR |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | Calvin University |
| Annotation Status | In GenBank |
| Plaque Notes | Plaques are small, circular, about 1 mm in diameter. They seem to have defined edges and are clear. |
| Morphotype | Siphoviridae |
| Number of Genes | 94 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | OR475276 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
162 |
| Pitt Freezer Box Grid# |
F4 |
| Available Files |
| GenBank File for Phamerator | Download |