| Detailed Information for Phage Pumpkiney |
| Discovery Information |
| Isolation Host | Gordonia terrae 3612 |
| Found By | Addison Brown and Elizabeth Wemhoff |
| Year Found | 2025 |
| Location Found | Crete, NE United States of America |
| Finding Institution | Doane University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Unknown |
| Isolation Temperature | Not entered |
| GPS Coordinates | 40.611843 N, 97.072492 W Map |
| Discovery Notes | Soil sample was collected by Ian Susa from a pumpkin field in Crete, Nebraska. |
| Naming Notes | The name Pumpkiney is because the soil sample this phage was extracted from came from the soil of a pumpkin farm. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 20, 2026 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina NextSeq 1000 |
| Read Type | Single-end reads |
| Read Length | 100 bp |
| Approximate Shotgun Coverage | 953 |
| Genome length (bp) | 57905 |
| Character of genome ends | Circularly Permuted |
| GC Content | 67.6% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | DE |
| Subcluster | DE4 |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | Doane University |
| Annotation Status | Being Annotated (Expected completion by 5/1/2026) |
| Plaque Notes | Plaques are small (about 0.5 mm) and very clear (lytic). |
| Morphotype | Siphoviridae |
| Number of Genes | 88 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Publication Info |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Not in Pitt Archives |
| Available Files |
| Plaque Picture | Download |
| EM Picture | Download |
