| Detailed Information for Phage Samba | |
| Discovery Information | |
| Isolation Host | Gordonia terrae 3612 |
| Found By | Jaclyn Hwang |
| Year Found | 2017 |
| Location Found | Pittsburgh, PA USA |
| Finding Institution | University of Pittsburgh |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 40.4423 N, 79.9529 W Map |
| Discovery Notes | Soil sample collected from a flower pot behind Asia Tea House in the Schenley Park on 9/19 at 9:20AM. The temperature was 64ยบ F and it had been sunny for most of the morning. The sample was carried around outside and into classrooms until in a book bag until taken to the lab at 3:30pm. |
| Naming Notes | One of my favorite latin dances. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Nov 30, 2017 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 1253 |
| Genome length (bp) | 51500 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | TCGCCGGTGA |
| GC Content | 67.2% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | CV |
| Subcluster | -- |
| Cluster Life Cycle | Temperate |
| Lysogeny Notes | No lysogens. |
| Other Cluster Members |
Click to ViewArchis Azula Barco Begonia BlingBling Blino Blueberry CaptainKirk2 CarolAnn Delian Fairfaxidum Fenry Frokostdame Gambino Guacamole Hitter Jalammah JasperJr JuJu KappaFarmDelta Lysidious Malachai Melba Mellie MintFen MissRona Murp Obliviate PCoral7 Petra PhrostedPhlake Pipp PrincePatrick Samba Toast UmaThurman Utz Walrus William Wisp Wocket Zarbodnamra ZiggyZoo |
| Annotating Institution | University of Pittsburgh |
| Annotation Status | In GenBank |
| Plaque Notes | This phage typically produces plaques that are typically small (2mm), circular, and clear with a faint halo around the edges of the plaque. The halo or cloudy area results from a combination of lytic and lysogenic infections. |
| Morphotype | Siphoviridae |
| Number of Genes | 79 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewSamba_1 Samba_2 Samba_3 Samba_4 Samba_5 Samba_6 Samba_7 Samba_8 Samba_9 Samba_10 Samba_11 Samba_12 Samba_13 Samba_14 Samba_15 Samba_16 Samba_17 Samba_18 Samba_19 Samba_20 Samba_21 Samba_22 Samba_23 Samba_24 Samba_25 Samba_26 Samba_27 Samba_28 Samba_29 Samba_30 Samba_31 Samba_32 Samba_33 Samba_34 Samba_35 Samba_36 Samba_37 Samba_38 Samba_39 Samba_40 Samba_41 Samba_42 Samba_43 Samba_44 Samba_45 Samba_46 Samba_47 Samba_48 Samba_49 Samba_50 Samba_51 Samba_52 Samba_53 Samba_54 Samba_55 Samba_56 Samba_57 Samba_58 Samba_59 Samba_60 Samba_61 Samba_62 Samba_63 Samba_64 Samba_65 Samba_66 Samba_67 Samba_68 Samba_69 Samba_70 Samba_71 Samba_72 Samba_73 Samba_74 Samba_75 Samba_76 Samba_77 Samba_78 Samba_79 |
| Submitted Minimal DNA Master File | Download |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MN096365 |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| SEA Lysate Titer | 4.33 x 10^10 pfu/mL |
| Pitt Freezer Box# | 58 |
| Pitt Freezer Box Grid# | A6 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
