| Detailed Information for Phage Samba |
| Discovery Information |
| Isolation Host | Gordonia terrae 3612 |
| Found By | Jaclyn Hwang |
| Year Found | 2017 |
| Location Found | Pittsburgh, PA USA |
| Finding Institution | University of Pittsburgh |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 40.4423 N, 79.9529 W Map |
| Discovery Notes | Soil sample collected from a flower pot behind Asia Tea House in the Schenley Park on 9/19 at 9:20AM. The temperature was 64ยบ F and it had been sunny for most of the morning. The sample was carried around outside and into classrooms until in a book bag until taken to the lab at 3:30pm. |
| Naming Notes | One of my favorite latin dances. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Nov 30, 2017 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 1253 |
| Genome length (bp) | 51500 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | TCGCCGGTGA |
| GC Content | 67.2% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | CV |
| Subcluster | -- |
| Cluster Life Cycle | Temperate |
| Lysogeny Notes | No lysogens. |
| Other Cluster Members |
|
| Annotating Institution | University of Pittsburgh |
| Annotation Status | In GenBank |
| Plaque Notes | This phage typically produces plaques that are typically small (2mm), circular, and clear with a faint halo around the edges of the plaque. The halo or cloudy area results from a combination of lytic and lysogenic infections. |
| Morphotype | Siphoviridae |
| Number of Genes | 79 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MN096365 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
4.33 x 10^10 pfu/mL |
| Pitt Freezer Box# |
58 |
| Pitt Freezer Box Grid# |
A6 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
