| Detailed Information for Phage Skysand |
| Discovery Information |
| Isolation Host | Gordonia terrae 3612 |
| Found By | Divinity Summers |
| Year Found | 2016 |
| Location Found | Williamsburg, VA USA |
| Finding Institution | College of William & Mary |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 37.270833 N, 76.7161 W Map |
| Discovery Notes | My phage was picked from David Hibbitts enrichment number 5 and it was picked on October 24, 2016. My DNA concentration was 159.3ng/ul.
His Phage, IJBrown has the same sequence. |
| Naming Notes | I named the Phage after my youngest sister Skylar and one of my favorite book characters named Rhysand. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Dec 20, 2016 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 1232 |
| Genome length (bp) | 67359 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGCCGCGTAC |
| GC Content | 65.5% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | CR |
| Subcluster | CR3 |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | College of William & Mary |
| Annotation Status | In GenBank |
| Plaque Notes | The capsid size is 58nm and the tail was around 250nm. Both were measured on a 100nm scale |
| Morphotype | Siphoviridae |
| Number of Genes | 95 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MH669013 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
121 |
| Pitt Freezer Box Grid# |
F9 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
