Gordonia phage Skysand
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Detailed Information for Phage Skysand
Discovery Information
Isolation HostGordonia terrae 3612
Found ByDivinity Summers
Year Found2016
Location FoundWilliamsburg, VA USA
Finding InstitutionCollege of William & Mary
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates37.270833 N, 76.7161 W Map
Discovery NotesMy phage was picked from David Hibbitts enrichment number 5 and it was picked on October 24, 2016. My DNA concentration was 159.3ng/ul.
His Phage, IJBrown has the same sequence.
Naming NotesI named the Phage after my youngest sister Skylar and one of my favorite book characters named Rhysand.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedDec 20, 2016
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina
Approximate Shotgun Coverage1232
Genome length (bp)67359
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceCGCCGCGTAC
GC Content65.5%
Fasta file available?Yes: Download fasta file
Characterization
ClusterCR
SubclusterCR3
Cluster Life CycleLytic
Other Cluster Members
Annotating InstitutionCollege of William & Mary
Annotation StatusIn GenBank
Plaque NotesThe capsid size is 58nm and the tail was around 250nm. Both were measured on a 100nm scale
MorphotypeSiphoviridae
Number of Genes95
Number of tRNAs0
Number of tmRNAs0
Has been Phamerated?Yes
Gene List

Click to View

Skysand_1

Skysand_2

Skysand_3

Skysand_4

Skysand_5

Skysand_6

Skysand_7

Skysand_8

Skysand_9

Skysand_10

Skysand_11
(HicA-like toxin)

Skysand_12

Skysand_13

Skysand_14

Skysand_15

Skysand_16

Skysand_17

Skysand_18

Skysand_19

Skysand_20
(lysin B)

Skysand_21

Skysand_22

Skysand_23

Skysand_24

Skysand_25
(HNH endonuclease)

Skysand_26
(terminase)

Skysand_27
(portal protein)

Skysand_28
(MuF-like minor capsid protein)

Skysand_29
(scaffolding protein)

Skysand_30
(LamD-like capsid decoration protein)

Skysand_31
(major capsid protein)

Skysand_32

Skysand_33
(head-to-tail adaptor)

Skysand_34

Skysand_35

Skysand_36

Skysand_37
(major tail protein)

Skysand_38

Skysand_39
(tail assembly chaperone)

Skysand_40
(tail assembly chaperone)

Skysand_41
(tape measure protein)

Skysand_42
(minor tail protein)

Skysand_43
(minor tail protein)

Skysand_44
(minor tail protein)

Skysand_45
(minor tail protein)

Skysand_46

Skysand_47

Skysand_48

Skysand_49

Skysand_50
(lysin A, protease C39 domain)

Skysand_51
(lysin A, glycosyl hydrolase domain)

Skysand_52

Skysand_53

Skysand_54

Skysand_55
(DNA primase/polymerase/helicase)

Skysand_56

Skysand_57
(WhiB family transcription factor)

Skysand_58

Skysand_59
(helix-turn-helix DNA binding protein)

Skysand_60
(RuvC-like resolvase)

Skysand_61
(DnaE-like DNA polymerase III)

Skysand_62

Skysand_63

Skysand_64

Skysand_65
(thioredoxin)

Skysand_66

Skysand_67
(RecA-like DNA recombinase)

Skysand_68
(Cas4 family exonuclease)

Skysand_69

Skysand_70

Skysand_71

Skysand_72

Skysand_73

Skysand_74

Skysand_75
(DNA helicase)

Skysand_76
(WhiB family transcription factor)

Skysand_77

Skysand_78

Skysand_79

Skysand_80

Skysand_81

Skysand_82

Skysand_83

Skysand_84

Skysand_85

Skysand_86

Skysand_87

Skysand_88

Skysand_89

Skysand_90

Skysand_91

Skysand_92

Skysand_93

Skysand_94

Skysand_95

Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMH669013
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 121
Pitt Freezer Box Grid# F9
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload