Detailed Information for Phage Walrus |
Discovery Information |
Isolation Host | Gordonia terrae 3612 |
Found By | Matthew Montgomery |
Year Found | 2016 |
Location Found | Pittsburgh, PA USA |
Finding Institution | University of Pittsburgh |
Program | Phage Hunters Integrating Research and Education |
From enriched soil sample? | No |
Isolation Temperature | 30°C |
GPS Coordinates | 40.442567 N, 79.957276 W Map |
Discovery Notes | This phage was a contaminant in a BetterKatz lysate. I isolated it by plating the contaminated lysate on the Betterkatz lysogen (so only the contaminant can infect). From there I purified and amplified using standard protocols. |
Naming Notes | Walruses are interesting animals. |
Sequencing Information |
Sequencing Complete? | Yes |
Date Sequencing Completed | Jan 20, 2017 |
Sequencing Facility | Pittsburgh Bacteriophage Institute |
Shotgun Sequencing Method | Illumina Sequencing |
Approximate Shotgun Coverage | 551 |
Genome length (bp) | 52024 |
Character of genome ends | 3' Sticky Overhang |
Overhang Length | 10 bases |
Overhang Sequence | TGGCCGGTGA |
GC Content | 67.3% |
Fasta file available? | Yes: Download fasta file |
Characterization |
Cluster | CV |
Subcluster | -- |
Cluster Life Cycle | Temperate |
Lysogeny Notes | Emperor is able to form a G. terrae 3612 lysogen. |
Other Cluster Members |
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Annotating Institution | Gettysburg College |
Annotation Status | In GenBank |
Plaque Notes | Plaques are small and turbid (may form lysogen). |
Morphotype | Siphoviridae |
Number of Genes | 84 |
Number of tRNAs | 0 |
Number of tmRNAs | 0 |
Has been Phamerated? | Yes |
Gene List |
|
Submitted Minimal DNA Master File | Download |
Publication Info |
Uploaded to GenBank? | Yes |
GenBank Accession | MK501729 |
Refseq Number | None yet |
Archiving Info |
Archiving status |
Archived |
Pitt Freezer Box# |
6 |
Pitt Freezer Box Grid# |
E8 |
Available Files |
Final DNAMaster File | Download |
GenBank File for Phamerator | Download |