Gordonia phage William
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Detailed Information for Phage William
Discovery Information
Isolation HostGordonia terrae 3612
Found ByParampreet Deol
Year Found2016
Location FoundPittsburgh, PA USA
Finding InstitutionUniversity of Pittsburgh
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates40.443383 N, 79.955009 W Map
Discovery NotesThe soil was collected from a flower bed outside a clear, sunny day. The soil was slightly moist, and I dug about 3 inches into the ground to collect it. Afterwards, it was stored in a refrigerator for about 1 day before being processed in lab.
Naming NotesThe name for this phage comes from the location it was found. It was found outside of the William Pitt Union at the University of Pittsburgh.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedFeb 16, 2017
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage456
Genome length (bp)50678
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceTCGCCGGTGA
GC Content66.5%
Sequencing NotesThere were two SNPs in this sample.

At position 23844, ~40% of the reads showed a G instead of the consensus A.
At position 24218, ~35% of the reads showed a G instead of the consensus A.
Fasta file available?Yes: Download fasta file
Characterization
ClusterCV
Subcluster--
Cluster Life CycleTemperate
Other Cluster Members
Annotating InstitutionUniversity of Pittsburgh
Annotation StatusIn GenBank
Plaque NotesWilliam produces clear plaques the relatively round and small, ranging from approximately 1 mm to 3 mm in length.
MorphotypeSiphoviridae
Number of Genes83
Number of tRNAs0
Number of tmRNAs0
Has been Phamerated?Yes
Gene List

Click to View

William_1
(terminase small subunit)

William_2
(terminase large subunit)

William_3
(portal protein)

William_4
(capsid maturation protease)

William_5
(scaffolding protein)

William_6
(major capsid protein)

William_7

William_8
(head-to-tail adaptor)

William_9
(head-to-tail stopper)

William_10

William_11

William_12

William_13
(tail terminator)

William_14
(major tail protein)

William_15
(tail assembly chaperone)

William_16
(tail assembly chaperone)

William_17
(tape measure protein)

William_18
(minor tail protein)

William_19
(minor tail protein)

William_20
(lysin A, L-Ala-D-Glu peptidase domain)

William_21
(lysin A, glycosyl hydrolase domain)

William_22

William_23

William_24

William_25

William_26
(lysin B)

William_27

William_28
(minor tail protein)

William_29
(minor tail protein)

William_30

William_31
(minor tail protein)

William_32
(minor tail protein)

William_33

William_34

William_35

William_36

William_37

William_38

William_39

William_40
(serine integrase)

William_41

William_42

William_43

William_44

William_45

William_46

William_47

William_48

William_49

William_50

William_51

William_52

William_53

William_54

William_55

William_56

William_57

William_58

William_59

William_60
(exonuclease)

William_61
(RecT-like ss DNA binding protein)

William_62

William_63

William_64

William_65

William_66

William_67

William_68

William_69

William_70

William_71

William_72

William_73

William_74

William_75

William_76

William_77

William_78

William_79
(RuvC-like resolvase)

William_80

William_81

William_82

William_83
(HNH endonuclease)

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMK801721
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 42
Pitt Freezer Box Grid# H12
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload