Gordonia phage William
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| Detailed Information for Phage William | |
| Discovery Information | |
| Isolation Host | Gordonia terrae 3612 |
| Found By | Parampreet Deol |
| Year Found | 2016 |
| Location Found | Pittsburgh, PA USA |
| Finding Institution | University of Pittsburgh |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 40.443383 N, 79.955009 W Map |
| Discovery Notes | The soil was collected from a flower bed outside a clear, sunny day. The soil was slightly moist, and I dug about 3 inches into the ground to collect it. Afterwards, it was stored in a refrigerator for about 1 day before being processed in lab. |
| Naming Notes | The name for this phage comes from the location it was found. It was found outside of the William Pitt Union at the University of Pittsburgh. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Feb 16, 2017 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 456 |
| Genome length (bp) | 50678 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | TCGCCGGTGA |
| GC Content | 66.5% |
| Sequencing Notes | There were two SNPs in this sample. At position 23844, ~40% of the reads showed a G instead of the consensus A. At position 24218, ~35% of the reads showed a G instead of the consensus A. |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | CV |
| Subcluster | -- |
| Cluster Life Cycle | Temperate |
| Other Cluster Members | |
| Annotating Institution | University of Pittsburgh |
| Annotation Status | In GenBank |
| Plaque Notes | William produces clear plaques the relatively round and small, ranging from approximately 1 mm to 3 mm in length. |
| Morphotype | Siphoviridae |
| Number of Genes | 83 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List | |
| Submitted Minimal DNA Master File | Download |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MK801721 |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| Pitt Freezer Box# | 42 |
| Pitt Freezer Box Grid# | H12 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
