| Detailed Information for Phage Charming |
| Discovery Information |
| Isolation Host | Gordonia terrae 3612 |
| Found By | Nicholas Johnbosco |
| Year Found | 2018 |
| Location Found | Pittsburgh, PA USA |
| Finding Institution | University of Pittsburgh |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 30°C |
| GPS Coordinates | 40.44803 N, 79.956289 W Map |
| Discovery Notes | The soil was collected from the Litchfield towers in Pittsburgh PA on August 20, 2018. The weather outside was cloudy and cool with the temperature being about 82 degrees at the time of collection, 3:37 PM. The soil sample was transported for 5 mins and placed in the cool dark drawer in my desk. The soil was cold and slightly damp. There were small trees and shrubs near the collection site. The area was quite shady due to the towers shadow. |
| Naming Notes | From a childhood fairy tale that I loved to read |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 15, 2019 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Approximate Shotgun Coverage | 1061 |
| Genome length (bp) | 58000 |
| Character of genome ends | Circularly Permuted |
| GC Content | 67.1% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | DE |
| Subcluster | DE1 |
| Cluster Life Cycle | Lytic |
| Lysogeny Notes | Phage Charming used a standard protocol to test for lysogeny which resulted in clearings all over the plate, which indicates a phage with a lytic cycle |
| Other Cluster Members |
|
| Annotating Institution | University of Pittsburgh |
| Annotation Status | In GenBank |
| Plaque Notes | Phage Charming produces circular plaques that are about 1-5mm in diameter with the majority of them being around the 3mm. |
| Morphotype | Siphoviridae |
| Number of Genes | 88 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MK937601 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
3 * 10^10 pfu/mL |
| Pitt Freezer Box# |
89 |
| Pitt Freezer Box Grid# |
H11 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
