| Detailed Information for Phage Love |
| Discovery Information |
| Isolation Host | Gordonia terrae 3612 |
| Found By | Hailey Malandra |
| Year Found | 2019 |
| Location Found | Pittsburgh, PA United States |
| Finding Institution | University of Pittsburgh |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 30°C |
| GPS Coordinates | 40.4421 N, 79.9563 W Map |
| Discovery Notes | I discovered this phage in very grainy/sandy soil. This whether was clear and dry but it had rained prior to the soil discovery so the soil was slightly damp. I collected the soil off of a very busy street at the University of Pittsburgh where there had been a water main break a few days prior. |
| Naming Notes | The name "Love" was chosen because I have a lot of love for this lab and I take care of this phage as if it were my child. Growing up my mother always called me Hailey-bug or Love-bug and I thought it was appropriate to name it "Love" since it is my love bug. Also I think it is cute. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 7, 2020 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Approximate Shotgun Coverage | 696 |
| Genome length (bp) | 60042 |
| Character of genome ends | Circularly Permuted |
| GC Content | 67.3% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | DE |
| Subcluster | DE1 |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | University of Pittsburgh |
| Annotation Status | In GenBank |
| Plaque Notes | The morphology of my phage's plaques were both small and large plaques even though many rounds of serial dilution we performed. The plaques were consistently clear as well. |
| Morphotype | Siphoviridae |
| Number of Genes | 91 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MT723934 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
7.7 x 10^10 |
| Pitt Freezer Box# |
114 |
| Pitt Freezer Box Grid# |
F3 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
