| Detailed Information for Phage MoontowerMania |
| Discovery Information |
| Isolation Host | Gordonia terrae CAG3 |
| Found By | Olivia Peoples & McKenzie Prunty |
| Year Found | 2022 |
| Location Found | Lawrence, KS United States of America |
| Finding Institution | Kansas State University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 21°C |
| GPS Coordinates | 38.83372 N, 95.25346 W Map |
| Discovery Notes | This sample was collected from the edge of the cornfield at our house. The soil this day was dry and sandy. I dug 1/2 inch down from the topsoil. |
| Naming Notes | We named this phage MoontowerMania. This is because our house was a very prominent party house. In "Dazed and Confused", the Moontower was the party house, so our house quickly became known as the moontower. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 9, 2023 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 1226 |
| Genome length (bp) | 59932 |
| Character of genome ends | Circularly Permuted |
| GC Content | 67.6% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | DE |
| Subcluster | DE1 |
| Cluster Life Cycle | Lytic |
| Lysogeny Notes | Clearing ere present during lysogenic patch test. Therefore, phage is a lysogen. |
| Other Cluster Members |
|
| Annotating Institution | Kansas State University |
| Annotation Status | In GenBank |
| Plaque Notes | round plaque with fuzzy edges |
| Number of Genes | 86 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | OR283206 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
6x10E10 pfu/mL |
| Pitt Freezer Box# |
160 |
| Pitt Freezer Box Grid# |
C6 |
| Available Files |
| Plaque Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
