| Detailed Information for Phage RoadKill |
| Discovery Information |
| Isolation Host | Gordonia terrae CAG3 |
| Found By | Lindsay Covell, Mikahla Searles, Liz Nadeau |
| Year Found | 2018 |
| Location Found | Farmington, ME USA |
| Finding Institution | University of Maine, Farmington |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 44.505928 N, 70.138217 W Map |
| Discovery Notes | This phage was isolated from a sewer ditch at the Farmington Wastewater Treatment Plant in Farmington, Maine. |
| Naming Notes | In our initial attempt to isolate a phage, we took samples from a dead squirrel that we found alongside a road. If successful in isolating a phage from this sample, we planned to name it RoadKill. Well, we didn't get a phage from that sample, so we're naming this one RoadKill in honor of the dead squirrel that helped us in our Phage Hunter's journey. Rest in peace, little squirrel. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 15, 2019 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 968 |
| Genome length (bp) | 55939 |
| Character of genome ends | Circularly Permuted |
| GC Content | 67.6% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | DE |
| Subcluster | DE3 |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | University of Maine, Farmington |
| Annotation Status | In GenBank |
| Plaque Notes | Clear, medium-sized, round plaques. |
| Number of Genes | 84 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MK977704 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
78 |
| Pitt Freezer Box Grid# |
A10 |
| Available Files |
| GenBank File for Phamerator | Download |