| Detailed Information for Phage Rofo |
| Discovery Information |
| Isolation Host | Gordonia terrae 3612 |
| Found By | Zoë Simpson |
| Year Found | 2016 |
| Location Found | Royersford, PA USA |
| Finding Institution | University of Pittsburgh |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 30°C |
| GPS Coordinates | 40.177419 N, 75.528453 W Map |
| Discovery Notes | Soil was collected from an area containing a lot of vegetation. The weather was warm but humid, and the soil was dry. |
| Naming Notes | "Rofo" is the nickname locals use for my hometown of Royersford, where this phage was discovered. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Feb 14, 2017 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 2204 |
| Genome length (bp) | 58767 |
| Character of genome ends | Circularly Permuted |
| GC Content | 67.3% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | DE |
| Subcluster | DE1 |
| Cluster Life Cycle | Lytic |
| Lysogeny Notes | Lysogen testing was not performed on Rofo. |
| Other Cluster Members |
|
| Annotating Institution | Gettysburg College |
| Annotation Status | In GenBank |
| Plaque Notes | Rofo produces one morphology: round plaques that are 1 mm in diameter. |
| Morphotype | Siphoviridae |
| Number of Genes | 85 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MH479924 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
1.83x10^-10 |
| Date of SEA Lysate Titering |
Oct 24, 2016 |
| Pitt Freezer Box# |
42 |
| Pitt Freezer Box Grid# |
G10 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
